@article{mbs:/content/journal/micro/10.1099/00221287-44-3-439, author = "Goldner, M. and Jakus, Cvetka M. and Rhodes, Harriett K. and Wilson, R. J.", title = "The Amino Acid Utilization by Phase I Bordetella pertussis in a Chemically Defined Medium", journal= "Microbiology", year = "1966", volume = "44", number = "3", pages = "439-444", doi = "https://doi.org/10.1099/00221287-44-3-439", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-44-3-439", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY: Phase I Bordetella pertussis was grown in a chemically defined medium containing 20 amino acids (including glutamine) plus glutathione. Growth was limited by depletion of three of the components, L-glutamic acid, L-proline and L-glutamine. Increasing the concentrations of these three components more than doubled the yield of organism. The following components were utilized, but did not limit growth: alanine, glycine, histidine, serine; cystine and glutathione were also substantially used. Ten amino acids which were not utilized to any significant extent could be omitted without diminishing growth. A preferential order of amino acid utilization is suggested. Glutamic acid appears to be important for antigenicity.", }