1887

Abstract

SUMMARY: Phase I was grown in a chemically defined medium containing 20 amino acids (including glutamine) plus glutathione. Growth was limited by depletion of three of the components, L-glutamic acid, L-proline and L-glutamine. Increasing the concentrations of these three components more than doubled the yield of organism. The following components were utilized, but did not limit growth: alanine, glycine, histidine, serine; cystine and glutathione were also substantially used. Ten amino acids which were not utilized to any significant extent could be omitted without diminishing growth. A preferential order of amino acid utilization is suggested. Glutamic acid appears to be important for antigenicity.

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/content/journal/micro/10.1099/00221287-44-3-439
1966-09-01
2024-04-23
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References

  1. Block R. J. 1950; Estimation of amino acids and amines on paper chromatograms.. Anal. Chem. 22:1327
    [Google Scholar]
  2. Block R. J., Durrum E. L., Zweig G. 1958; Quantitative methods.. A Manual of Paper Chromatography and Paper Electrophoresis85–109 New York: Academic Press, Inc.;
    [Google Scholar]
  3. Cohen S. M., Wheeler M. W. 1946; Pertussis vaccine prepared with phase-1 cultures grown in fluid medium.. Am. J. pub. Hlth 36:371
    [Google Scholar]
  4. Fukumi H., Sayama E., Tomizawa J., Uchida T. 1953; Nutritional requirements and respiratory pattern of pertussis-parapertussis-bronchisepticus group of micro-organisms.. Jap. J. med. Sci. Biol. 6:587
    [Google Scholar]
  5. Hanes C. S., Harris C. K., Moscarello M. A., Tigane E. 1961; Quantitative chromatographic methods. Part 4. Stabilized chromatographic systems of high resolving power for amino acids.. Can. J. Biochem. Physiol. 39:163
    [Google Scholar]
  6. Jebb W. H. H., Tomlinson A. H. 1951; The catabolic activity of washed suspensions of Haemophilus pertussis.. J. gen. Microbiol. 5:951
    [Google Scholar]
  7. Jebb W. H. H., Tomlinson A. H. 1955; The nutritional requirements of Haemophilus pertussis.. J. gen. Microbiol. 13:1
    [Google Scholar]
  8. Rowatt E. 1957a; The growth of Bordetella pertussis: a review.. J. gen. Microbiol. 17:297
    [Google Scholar]
  9. Rowatt E. 1957b; Some factors affecting the growth of Bordetella pertussis.. J. gen. Microbiol. 17:279
    [Google Scholar]
  10. Slotta K. H., Primosigh J. 1951; Amino-acid composition of crotoxin.. Nature, Lond. 168:696
    [Google Scholar]
  11. Ungar J., James A. M., Muggleton P. W., Pegler H. F., Tomich E. G. 1950; The cultivation of Haemophilus pertussis in partially-defined liquid media.. J. gen. Microbiol. 4:345
    [Google Scholar]
  12. Vajdic A. H., Goldner M., Wilson R. J. 1966; The amino acid used in cultures of phase 1 Bordetella pertussis during growth in chemically defined media.. J. gen. Microbiol. 44:445
    [Google Scholar]
  13. Wade E. H. M., Matheson A. T., Hanes C. S. 1961; Quantitative chromatographic methods. Part 3. Factors controlling the patterns of separation of the amino acids on paper chromatograms.. Can. J. Biochem. Physiol. 39:141
    [Google Scholar]
  14. Wilson R. J. 1963; Cultivation of Bordetella pertussis (phase 1) in a chemically-defined medium.. Can. J. pub. Hlth 54:518
    [Google Scholar]
  15. Worcester J., Wilson E. B. 1943; A table determining LD 50 or the fifty percent end-point.. Proc. natn. Acad. Sci., U.S.A. 29:207
    [Google Scholar]
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