1887

Abstract

SUMMARY: Preparations of flocculent and of non-flocculent cell walls were obtained from flocculent and non-flocculent cells of a strain of Flocculent walls contained 46% glucan, 43% mannan, 0.4% P, 1.1% hexosamine, 0.79% non-hexosamine N; non-flocculent walls contained 47% glucan, 44% mannan, 0.3% P, 1.2% hexosamine and 0.98%non-hexosamine N.

Mannose-6-phosphate was identified as the principal phosphorus compound present. The mannose residue formed part of the cell-wall mannan and the phosphate was also linked by a second, labile, ester bond to an unidentified site. The degree of phosphorylation of the mannan varied from 1 phosphate to 19 mannose residues in non-flocculent cells to 1 phosphate to 13 residues in flocculent cells.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-44-3-329
1966-09-01
2021-08-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/44/3/mic-44-3-329.html?itemId=/content/journal/micro/10.1099/00221287-44-3-329&mimeType=html&fmt=ahah

References

  1. Boas N. F. 1953; Method for the determination of hexosamines in tissues.. J. biol.chem. 204:553
    [Google Scholar]
  2. Badin J., Jackson C., Schubert M. 1957; Improved method for determination of plasma polysaccharides with tryptophan.. Proc. Soc. exp. Biol. Med. 84:288
    [Google Scholar]
  3. Conway E. G. 1957 Microdiffusion Analysis and Volumetric Error. London: Lockwood and Son Ltd.;
    [Google Scholar]
  4. Eddy A. A. 1958; The structure of the yeast cell wall. II. Degradative studies with enzymes.. Proc. roy. Soc. B 149:425
    [Google Scholar]
  5. Falcone G., Nickerson W. J. 1956; Cell-wall mannan-protein of baker’s yeast.. Science 124:272
    [Google Scholar]
  6. Fiske C. H., Subbarow Y. 1925; The colorimetric determination of phosphorus.. J. biol. Chem. 66:375
    [Google Scholar]
  7. Fleury P. F., Lange J. 1933; Determination of periodic acid in the presence of iodic acid.. J. Pharm. Chim., Paris 17:107
    [Google Scholar]
  8. Hanes C., Isherwood S, F. A. 1949; Separation of the phosphoric esters on the filter paper chromatogram. Nature, Lond. 164:1107
    [Google Scholar]
  9. Haworth W., Heath R. L., Peat S. 1941; Constitution of yeast mannan.. J. chem. Soc.833
    [Google Scholar]
  10. Haworth W. N., Hirst E. L., Isherwood F. A. 1937; Polysaccharides. XXIV. Yeast mannan.. J. chem. Soc.784
    [Google Scholar]
  11. Horecker B. L., Wood W. A. 1957; d-glucose-6-phosphate.. Meth. Enzym. 3:153
    [Google Scholar]
  12. Jeanes A., Pittsley J. E., Watson P. R., Dimler R. J. 1961; Characterization and properties of the phosphomannan from Hansenula holstii nrrl-y-2448.. Arch. Biochem. Biophys. 92:343
    [Google Scholar]
  13. Kaplan O. N. 1957; Enzymatic determination of free sugars.. Meth. Enzym. 3:107
    [Google Scholar]
  14. Korn E. D., Northcote H. 1960; Physical and chemical properties of polysaccharides and glycoproteins of the yeast cell wall.. Biochem. J. 75:12
    [Google Scholar]
  15. Leloir L. F., Cardini C. E. 1957; Characterization of phosphorus compounds by acid lability.. Meth. Enzym. 3:840
    [Google Scholar]
  16. Masschelein C. A. 1959; Structure de la paroi cellulaire et mécanisme de la flocculence des levures bases.. Revue-Ferment. Ind. aliment. 14:59
    [Google Scholar]
  17. Masschelein C. A., Devreux A. 1957; Flocculence et structure de la paroi cellulaire de la levure.. Proc. Eur. Brew. Conv.194
    [Google Scholar]
  18. Masschelein C. A., Jeunehomme-Ramos C., Castiau C., Devreux A. 1963; Mechanism of phenotypic variations in the flocculence character of yeast.. J. Inst. Brew. 69:332
    [Google Scholar]
  19. Mill P. J. 1964; The effect of nitrogenous substances on the time of flocculation of Saccharomyces cerevisiae.. J. gen. Microbiol. 35:53
    [Google Scholar]
  20. Nelson N. 1944; Photometric adaptation of the Somogyi method for the determination of glucose.. J. biol. Chem. 153:375
    [Google Scholar]
  21. Northcote D. H., Horne R. W. 1952; The chemical composition and structure of the yeast cell wall.. Biochem. J. 51:232
    [Google Scholar]
  22. Peat S., Turvey J. R., Doyle D. 1961; The polysaccharides of bakers’ yeast.V. A further study of the mannan.. J. chem. Soc.3918
    [Google Scholar]
  23. Roelofsen P. A. 1953; Yeast mannan, a cell wall constituent of bakers’ yeast.. Biochim. Biophys. Acta 10:477
    [Google Scholar]
  24. Scott T. A., Melvin E. H. 1953; Determination of dextran with anthrone.. Anal. chem. 25:1656
    [Google Scholar]
  25. Slodki M. E. 1962; Phosphate linkages in phosphomannans from yeast.. Biochim. biophys. Acta 57:525
    [Google Scholar]
  26. Speck J. C. 1962; Determination of formaldehyde.. Meth. carbohyd. Chem. 1:441
    [Google Scholar]
  27. Trevelyan W. E., Harrison J. S. 1952; Yeast metabolism. 1. Fractionation and microdetermination of cell carbohydrates.. Biochem. J. 50:298
    [Google Scholar]
  28. Trevelyan W. E., Harrison J. S. 1956; Yeast metabolism. 7. Yeast carbohydrate fractions, separation from nucleic acid, analysis, and behaviour during fermentation.. Biochem. J. 63:23
    [Google Scholar]
  29. Trevelyan W. E., Procter D. P., Harrison J. S. 1950; Detection of sugars on paper chromatograms.. Nature, Lond. 166:444
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-44-3-329
Loading
/content/journal/micro/10.1099/00221287-44-3-329
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error