1887

Abstract

SUMMARY: The uptake of aliphatic amides by 8602 was studied by using 1-C-acetamide and 1-C--acetylacetamide (a non-metabolizable analogue). 1-C-acetamide was accumulated by the wild-type strains and by an amidase-negative mutant. The maximum ratio of internal to external concentration measured was 100:1. 1-C--acetylacetamide was concentrated by the wild-type strain and the maximum ratio of internal to external concentration measured was 80:1. No difference was detected in amide uptake as between induced and non-induced cultures of the wild-type or mutant strains. It is concluded that the organism possesses a constitutative permease for these amides. Cyano-acetamide had no significant effect on -acetylacetamide accumulation by the wild-type strain at concentrations which repressed amidase synthesis. Repression of amidase synthesis by amide analogue repressors is therefore not due to inhibition of amide uptake by the bacteria.

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/content/journal/micro/10.1099/00221287-44-2-303
1966-08-01
2021-08-06
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References

  1. Barrett J. T., Kallio R. E. 1953; Terminal respiration in Pseudomonas fluorescens. Component enzymes of the tricarboxylic acid cycle.. J. Bact. 66:517
    [Google Scholar]
  2. Brammar W. J., Clarke P. H. 1964; Induction and repression of Pseudomonas aeruginosa amidase.. J. gen. Microbiol 37:307
    [Google Scholar]
  3. Clarke P. H., Brammar W. J. 1964; Regulation of bacterial enzyme synthesis by induction and repression.. Nature, Lond. 203:1153
    [Google Scholar]
  4. Clarke P. H., Meadow P. M. 1959; Evidence for the occurrence of permeases for tricarboxylic acid intermediates in Pseudomonas aeruginosa . J. gen. Microbiol. 20:144
    [Google Scholar]
  5. Cohen G. N., Monod J. 1957; Bacterial permeases.. Bact. Rev. 21:169
    [Google Scholar]
  6. Fox C. F., Kennedy E. P. 1965; Specific labelling and partial purification of the M protein, a component of the β-galactoside transport system of Escherichia coli.. Proc. natn. acad. Sci., U.S.A. 54:891
    [Google Scholar]
  7. Horecker B. L., Thomas J., Monod J. 1960; Galactose transport in Escherichia coli. 1. General properties as studied in a galactokinase-less mutant.. J. biol. Chem. 235:1580
    [Google Scholar]
  8. Jacob F., Monod J. 1961; Genetic regulatory mechanisms in the synthesis of proteins.. J. mol. Biol. 3:318
    [Google Scholar]
  9. Kepes A., Cohen G. N. 1962; Permeation. The Bacteria 4:199 New York: Academic Press.;
    [Google Scholar]
  10. Kogut M., Podoski E. P. 1953; Oxidative pathways in a fluorescent Pseudomonas . Biochem. J. 55:800
    [Google Scholar]
  11. Skinner A. J., Clarke P. H. 1965; C2 mutants of Pseudomonas aeruginosa.. J. gen. Microbiol. 39:viii
    [Google Scholar]
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