SUMMARY: The uptake of aliphatic amides by 8602 was studied by using 1-C-acetamide and 1-C--acetylacetamide (a non-metabolizable analogue). 1-C-acetamide was accumulated by the wild-type strains and by an amidase-negative mutant. The maximum ratio of internal to external concentration measured was 100:1. 1-C--acetylacetamide was concentrated by the wild-type strain and the maximum ratio of internal to external concentration measured was 80:1. No difference was detected in amide uptake as between induced and non-induced cultures of the wild-type or mutant strains. It is concluded that the organism possesses a constitutative permease for these amides. Cyano-acetamide had no significant effect on -acetylacetamide accumulation by the wild-type strain at concentrations which repressed amidase synthesis. Repression of amidase synthesis by amide analogue repressors is therefore not due to inhibition of amide uptake by the bacteria.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error