1887

Abstract

SUMMARY: The uptake of aliphatic amides by 8602 was studied by using 1-C-acetamide and 1-C--acetylacetamide (a non-metabolizable analogue). 1-C-acetamide was accumulated by the wild-type strains and by an amidase-negative mutant. The maximum ratio of internal to external concentration measured was 100:1. 1-C--acetylacetamide was concentrated by the wild-type strain and the maximum ratio of internal to external concentration measured was 80:1. No difference was detected in amide uptake as between induced and non-induced cultures of the wild-type or mutant strains. It is concluded that the organism possesses a constitutative permease for these amides. Cyano-acetamide had no significant effect on -acetylacetamide accumulation by the wild-type strain at concentrations which repressed amidase synthesis. Repression of amidase synthesis by amide analogue repressors is therefore not due to inhibition of amide uptake by the bacteria.

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/content/journal/micro/10.1099/00221287-44-2-303
1966-08-01
2020-01-24
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-44-2-303
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