Some Properties of Three Related Viruses: Andean Potato Latent, Dulcamara Mottle, and Ononis Yellow Mosaic Free

Abstract

SUMMARY: Three similar and apparently previously unrecorded viruses were studied and found to be most like viruses of the turnip yellow mosaic group. Andean potato latent virus (APLV) was obtained from primitive cultivated potatoes collected in the high tropical Andes, dulcamara mottle virus (DMV) from L. growing near Rothamsted, and Ononis yellow mosaic virus from L. growing in many parts of England. All three viruses are readily transmitted by sap inoculation; APLV and DMV are transmitted through the seed of infected plants; DMV is transmitted by the flea beetle Paykull. Plants infected by one of the viruses are not protected against infection by the others.

Purified preparations of these viruses have many common properties. Each virus has isometric particles 25-30 mμ in diameter, indistinguishable in appearance from one another and from the particles of turnip yellow mosaic virus. Preparations of each contain mainly two types of particles with sedimentation coefficients of about 115 and 55, corresponding to infective nucleoprotein particles and non-infective “empty” particles respectively. Each contains nucleic acid with a molar base composition of about G 16% A 22% C 33% U 29%. The three viruses are serologically related; antisera titres are 8-128 times greater with homologous than with the heterologous viruses. No serological relationship was found between these viruses and turnip yellow mosaic, wild cucumber mosaic, cocoa yellow mosaic, squash mosaic and red clover mottle viruses.

Plants infected with APLV or DMV when sprayed with solutions of 2-thiouracil or 6-azauracil produced fewer nucleoprotein particles and more “empty” protein particles, than plants sprayed with water.

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1966-08-01
2024-03-28
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References

  1. Agrawal H. 1964; Identification of cowpea mosaic virus isolates.. Meded. LandHogesch. Wageningen64
    [Google Scholar]
  2. Bancroft J. B. 1962; Purification and properties of bean pod mottle virus and associated centrifugal and electrophoretic components.. Virology, 16:419
    [Google Scholar]
  3. Bawden F. C. 1950 Plant Viruses and Virus Diseases 3rd ed. Waltham, Mass., U.S.A.: Chronica Botanica.;
    [Google Scholar]
  4. Bos L., Hagedorn D. J., Quantz L. 1960; Suggested procedures for international identification of legume viruses.. Tijdschr. PlZiekt. 66:328
    [Google Scholar]
  5. Brunt A. A., Kenten R. H., Gibbs A. J., Nixon H. L. 1965; Further studies on cocoa yellow mosaic virus.. J. gen. Microbiol. 38:81
    [Google Scholar]
  6. Campbell R. N. 1964; Radish mosaic virus, a crucifer virus serologically related to strains of bean pod mottle virus and to squash mosaic virus.. Phytopathology, 54:1418
    [Google Scholar]
  7. Dodds K. S. 1962; Classification of cultivated potatoes. The potato and its wild relatives. Ed. by Correll D. S. Renner, Texas: Texas Research Foundation.;
    [Google Scholar]
  8. Dorner R. W., Knight C. A. 1953; The preparation and properties of some plant virus nucleic acids.. J. biol. Chem. 205:959
    [Google Scholar]
  9. Francki R. I. B., Matthews R. E. F. 1962; Some effects of 2-thiouraeil on the multiplication of turnip yellow mosaic virus.. Virology, 17:367
    [Google Scholar]
  10. Freitag J. H. 1952; Seven virus diseases of cucurbits in California.. Phytopathology (abstr.), 42:8
    [Google Scholar]
  11. Huxley H. E., Zubay G. 1960; The structure of the protein shell of turnip yellow mosaic virus.. J. mol. Biol. 2:180
    [Google Scholar]
  12. John Innes Institute 1960 Ann. Rep. Innes Inst. 51:26
    [Google Scholar]
  13. John Innes Institute1 1963 Ann. Rep. Innes Inst. 54:37
    [Google Scholar]
  14. Katz S. 1962; Locating ultraviolet-absorbing substances on paper chromatographs.. J. chem. Educ. 39:34
    [Google Scholar]
  15. MacLeod R., Markham R. 1963; Experimental evidence of a relationship between turnip yellow mosaic virus and wild cucumber mosaic virus.. Virology, 19:190
    [Google Scholar]
  16. Mansi W. 1958; Slide gel diffusion precipitin test.. Nature, Lond. 181:1289
    [Google Scholar]
  17. Markham R. 1955 Modern Methods of Plant Analysis. Ed. by Paech K., Tracey M. V. 4:246
    [Google Scholar]
  18. Markham R. 1959 The Viruses. Ed. by Burnet F. M., Stanley W. M. 2chap. 2 New York and London: Academic Press.;
    [Google Scholar]
  19. Markham R. 1960; A graphical method for the rapid determination of sedimentation coefficients.. Biochem. J. 77:516
    [Google Scholar]
  20. Markham R., Smith J. D. 1951; Chromatographic studies of nucleic acids.. Biochem. J. 49:401
    [Google Scholar]
  21. Markham R., Smith K. M. 1949; Studies on the virus of turnip yellow mosaic.. Parasitology, 39:330
    [Google Scholar]
  22. Martini C. 1957; The transmission of turnip viruses by biting insects and aphids.. Proc. 3rd Conf. Potato Virus Diseases106
    [Google Scholar]
  23. Mazzone H. M., Incardona N. L., Kaesberg P. 1962; Biochemical and biophysical properties of squash mosaic virus and related macromolecules.. Biochim. biophys. Acta, 55:164
    [Google Scholar]
  24. McKee R. K. 1964; Virus infection in South American potatoes.. Eur. Potato J. 7:145
    [Google Scholar]
  25. Miller G. L., Price W. C. 1946; Physical and chemical studies on southern bean mosaic virus.. Archiv. Biochem. 10:467
    [Google Scholar]
  26. Nixon H. L., Gibbs A. J. 1960; Electron microscope observations on the structure of turnip yellow mosaic virus. J.. mol. Biol. 2:197
    [Google Scholar]
  27. Nixon H. L., Harrison B. D. 1959; Electron microscopic evidence on the structure of tobacco rattle virus.. J. gen. Microbiol. 21:582
    [Google Scholar]
  28. Perring F. H., Walters S. M. 1962 Atlas of the British Flora. London: Thomas Nelson.;
    [Google Scholar]
  29. Reichmann M. E. 1965; Determination of ribonucleic acid content of spherical viruses from sedimentation coefficients of full and empty particles.. Virology 25:166
    [Google Scholar]
  30. Ross J. P. 1963; Transmission of bean pod mottle virus in soybeans by beetles.. Pl. Dis. Reptr, 47:1049
    [Google Scholar]
  31. Schuster M. F. 1963; Flea beetle transmission of tobacco ringspot virus in the lower Rio Grande Valley.. Pl. Dis. Reptr, 47:510
    [Google Scholar]
  32. Semancik J. S., Bancroft J. B. 1964; Further characterization of the nucleoprotein components of bean pod mottle virus.. Virology, 22:33
    [Google Scholar]
  33. Shepherd R. J. 1963; Serological relationship between bean pod mottle virus and cowpea mosaic virus from Arkansas and Trinidad.. Phytopathology, 53:865
    [Google Scholar]
  34. Sinha R. C. 1960; Red clover mottle virus.. Ann. appl. Biol. 48:742
    [Google Scholar]
  35. Stace-Smith R., Reichmann M. E., Wright N. S. 1965; Purification and properties of tobacco ringspot virus and two RNA-deflcient components.. Virology 25:487
    [Google Scholar]
  36. Steere R. L. 1956; Purification and properties of tobacco ringspot virus.. Phytopathology, 46:60
    [Google Scholar]
  37. Symons R. H., Rees M. W., Short M. N., Markham R. 1963; Relationships between the ribonucleic acid and protein of some plant viruses.. J. mol. Biol. 6:1
    [Google Scholar]
  38. Walters H. J. 1964a; Transmission of bean pod mottle virus by bean leaf beetles.. Phytopathology, 54:240
    [Google Scholar]
  39. Walters H. J. 1964b; Transmission of southern bean mosaic virus by the bean leaf beetle.. Pl. Dis. Reptr 48:935
    [Google Scholar]
  40. Yamazaki H., Kaesberg P. 1961; Biophysical and biochemical properties of wild cucumber mosaic virus and two related virus-like particles.. Biochim. biophys. Acta, 51:9
    [Google Scholar]
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