Summary: An investigation of the effects on of crude and a highly purified tetanus neurotoxin preparation free from oxidase and haemolysin activity was made to assess the value of this organism in a bioassay system and as a convenient organism for the study of the mode of action of the toxin. Whereas crude tetanus toxin produced cytotoxic changes in purified tetanus neurotoxin did not even when the paramecia were exposed to ten human lethal doses.

Incubation of the paramecia with fluorescent-labelled toxin revealed that it accumulated within food vacuoles and in focal areas of cytoplasm but not in the cell membrane. Cytologic studies of the paramecia by means of light and electron microscopy revealed no abnormalities.


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