1887

Abstract

Summary: An investigation of the effects on of crude and a highly purified tetanus neurotoxin preparation free from oxidase and haemolysin activity was made to assess the value of this organism in a bioassay system and as a convenient organism for the study of the mode of action of the toxin. Whereas crude tetanus toxin produced cytotoxic changes in purified tetanus neurotoxin did not even when the paramecia were exposed to ten human lethal doses.

Incubation of the paramecia with fluorescent-labelled toxin revealed that it accumulated within food vacuoles and in focal areas of cytoplasm but not in the cell membrane. Cytologic studies of the paramecia by means of light and electron microscopy revealed no abnormalities.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-44-1-89
1966-07-01
2021-08-03
Loading full text...

Full text loading...

/deliver/fulltext/micro/44/1/mic-44-1-89.html?itemId=/content/journal/micro/10.1099/00221287-44-1-89&mimeType=html&fmt=ahah

References

  1. Beale G. H., Kacser H. 1957; Studies on the antigens of Paramecium aurelia with the aid of fluorescent antibodies. J. gen. Microbiol 17:68
    [Google Scholar]
  2. Rinderknecht H. 1962; Ultra rapid fluorescent labelling of proteins. Nature, Lond 193:167
    [Google Scholar]
  3. Roux J., Serre A. 1962; Action de la toxine tétanique sur la cellule de protozaire.Etudes sur Paramecium aurelia . Ann. Inst. Pasteur 103:623
    [Google Scholar]
  4. Roux J., Serre A. 1964a; Action de la toxine tetanique sur Paramecium aurelia. Facteurs influencant l’action de la toxine et réversibilitie de la fixation. Ann. Inst. Pasteur 107:635
    [Google Scholar]
  5. Roux J., Serre A. 1964b; Obtention de lignes de Paramecies sensibilises a la toxine tetanique. Ann. Inst. Pasteur 107:647
    [Google Scholar]
  6. Sheff M. F., Perry M. B., Zacks S. I. 1965; Studies on tetanus toxin II. The preparation of purified tetanus toxin and its fluorescent labelling. Biochim. biophys. Acta 100:215
    [Google Scholar]
  7. Sonneborn T. M. 1947; Recent advances in the genetics of Paramecium and Euplotes. Advanc. Genet 1:264
    [Google Scholar]
  8. Zacks S. I., Sheff N. F. 1964; Studies on tetanus toxin I. Formation of intramitochondrial dense granules in mice acutely poisoned with tetanus toxin. J. Neuropath, exp. Neurol 23:306
    [Google Scholar]
  9. Zacks S. I., Sheff M. F. 1965; Studies on tetanus toxin III. Intercellular localization of fluorescent-labelled tetanus toxin and antitoxin in mice. Acta Neuropath 4:267
    [Google Scholar]
  10. Zacks S. I., Hall J. A. S., Sheff M. F. 1966; Studies on tetanus IV. Intramatichondrial dense granules in skeletal muscle from human cases of chronic tetanus intoxication. Am. J. Path (in the Press)
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-44-1-89
Loading
/content/journal/micro/10.1099/00221287-44-1-89
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error