1887

Abstract

Summary: The BHK21-C13 line of hamster fibroblasts was infected with human parainfluenza virus type 2. The infection persisted through 40 subcultures without affecting the plating efficiency of the cells and without degeneration of the monolayers. Although all cells contained fluorescent-staining virus antigen and most became specifically haemadsorbing and contained inclusion bodies, the titre of virus in 4-day-old cultures was only equivalent to 0.0001 plaque-forming unit/cell. Infection of cells continued when clones were grown in anti-parainfluenzal serum. There was only very slight interference to infection with other viruses and superinfection with parainfluenza type 2 virus caused degenration of the culture. Persistent infection of BHK21 cells with human type 2 parainfluenza virus producedno loss of contact inhibition and no ability to grow in semi-solid media, changes which are characteristic of BHK21 cells when transformed by polyoma virus of Rous sarcoma virus.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-44-1-47
1966-07-01
2024-10-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/44/1/mic-44-1-47.html?itemId=/content/journal/micro/10.1099/00221287-44-1-47&mimeType=html&fmt=ahah

References

  1. Fernandes M. V., Wiktor T. J., Koprowski H. 1964; Endosymbiotic relationship between animal viruses and host cells. A study of rabies virus in tissue culture. J. exp. Med 120:1099
    [Google Scholar]
  2. Fraser K. B., Gharpure M. 1962; Immunofluorescent tracing of polyoma virus in transformation experiments with BHK21 cells. Virology 18:505
    [Google Scholar]
  3. Gharpure M. 1965; A heat-sensitive cellular function required for the replication of DNA viruses, but not RNA viruses. Virology 27:308
    [Google Scholar]
  4. Ginsberg H. S. 1958; The significance of the viral carrier state in tissue culture systems. Prog. med. Virol 1:36
    [Google Scholar]
  5. Gresser I., Enders J. F. 1961; A note on the presence of inclusion bodies in dividing human kidney cells infected with croup-associated virus. Virology 13:370
    [Google Scholar]
  6. Hare D. J., Morgan H. B. 1964; Polyoma virus and L cell relationship. II. A curable carrier system not dependent on interferon. J. natn. Cancer Inst 33:765
    [Google Scholar]
  7. Henle G., Hinze H. C., Henle W. 1963; Persistent infection of L cells with polyoma virus: periodic destruction and repopulation of the cultures. J. natn. Cancer Inst 31:125
    [Google Scholar]
  8. Isaacs A. 1963; Interferon. Adv. Virus Res 10:1
    [Google Scholar]
  9. Macpherson I. 1965; Reversion in hamster cells transformed by Rous sarcoma virus. Science 148:1731
    [Google Scholar]
  10. Macpherson I., Montagnier L. 1964; Agar suspension culture for the selective assay of cells transformed by polyoma virus. Virology 23:291
    [Google Scholar]
  11. Macpherson I., Stoker M. 1962; Polyoma transformation of hamster cell clones—an investigation of genetic factors affecting cell competence. Virology 16:147
    [Google Scholar]
  12. Rustigian R. 1962; A carrier state in HeLa cells with measles virus (Edmonston strain) apparently associated with non-infectious virus. A preliminary report. Virology 16:101
    [Google Scholar]
  13. Stoker M., Macpherson I. 1964; Syrian hamster fibroblast cell line BHK21 and its derivatives. Nature, Lond 203:1355
    [Google Scholar]
  14. Tyrrell D. A. J. 1959; Interferon produced by cultures of calf kidney cells. Nature, Lond 184:452
    [Google Scholar]
  15. Vogel J., Shelokov A. 1957; Adsorption-hemagglutinin test for influenza virus in monkey kidney tissue culture. Science 126:358
    [Google Scholar]
  16. Walker D. L., Hinze H. C. 1962a; A carrier state of mumps virus in human conjuctiva cells. I. General characteristics. J. exp. Med 116:739
    [Google Scholar]
  17. Walker D. L., Hinze H. C. 1962b; A carrier state of mumps virus in human conjuctiva cells. II. Observations on intracellular transfer of virus and virus release. J. exp. Med 116:751
    [Google Scholar]
/content/journal/micro/10.1099/00221287-44-1-47
Loading
/content/journal/micro/10.1099/00221287-44-1-47
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error