Summary: The BHK21-C13 line of hamster fibroblasts was infected with human parainfluenza virus type 2. The infection persisted through 40 subcultures without affecting the plating efficiency of the cells and without degeneration of the monolayers. Although all cells contained fluorescent-staining virus antigen and most became specifically haemadsorbing and contained inclusion bodies, the titre of virus in 4-day-old cultures was only equivalent to 0.0001 plaque-forming unit/cell. Infection of cells continued when clones were grown in anti-parainfluenzal serum. There was only very slight interference to infection with other viruses and superinfection with parainfluenza type 2 virus caused degenration of the culture. Persistent infection of BHK21 cells with human type 2 parainfluenza virus producedno loss of contact inhibition and no ability to grow in semi-solid media, changes which are characteristic of BHK21 cells when transformed by polyoma virus of Rous sarcoma virus.


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