1887

Abstract

Summary: L forms were produced by the penicillin gradient plate technique from four strains of . All strains were group B and had various sulphadiazine sensitivities. One parent strain had been propagated for many years in the laboratory, whereas the other three were isolated from recent cases of meningitis. To date, each L form has had more than 60 serial passages on medium containing penicillin. From two strains stabilized L form variants developed on penicillin-free medium following 30 or 40 such passages. Morphological characteristics of these organisms were similar to L forms of other bacteria. Medium and environmental conditions necessary for optimal growth included: brain heart infusion containing a final agar concentration of 1.2% (w/v), sucrose, 10% (w/v), horse serum, 10% (v/v), pH 7.4, temperature 37° and increased CO tension (candle jar). The L forms were non-groupable and had various fermentative reactions, whereas their sensitivity to sulphadiazine was the same as that of their respective parents. Revertants were produced on penicillin-free medium following every tenth serial passage in the L-form state. Sensitivities to sulphadiazine, fermentative reactions and serological groups of the revertant strains were identical with those of their respective parents.

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/content/journal/micro/10.1099/00221287-44-1-139
1966-07-01
2022-01-27
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References

  1. Bristow W. M., VanPeenen P. F. D., Volk R. 1965; Epidemic meningitis in naval recruits. Am. J. publ. Hlth 55:1039
    [Google Scholar]
  2. Brown J. W., Condit P. K. 1965; Meningococcal infections. Fort Ord and California. Calif. Med 102:171
    [Google Scholar]
  3. Catlin B. W., Cunningham L. S. 1961; Transforming activities and base contents of deoxyribonucleate preparations from various Neisseriae. J. gen. Microbiol 26:303
    [Google Scholar]
  4. Communicable Disease Center 1964 Morbidity and Mortality, weekly report 13:438
    [Google Scholar]
  5. Dienes L., Weinberger H. J. 1951; The L forms of bacteria. Bact. Rev 15:245
    [Google Scholar]
  6. Dienes L., Bandur B. M., Madoff S. 1964; Development of L-type growth in Neisseria gonorrhoeae cultures. J. Bact 87:1471
    [Google Scholar]
  7. Edward D. G. ff 1950; An investigation of the biological properties of organisms of the pleuropneumonia group, with suggestions regarding the identification of strains. J. gen. Microbiol 4:311
    [Google Scholar]
  8. Edward D. G. ff 1953; A difference in growth requirements between bacteria in the L-phase and organisms of the pleuropneumonia group. J. gen. Microbiol 8:256
    [Google Scholar]
  9. Evans J. R., Hunter D. H., Cary S. G., Rust J. H. 1964; Simplified method for determination of sulfadiazine sensitivity and fermentation reactions of Neisseria meningitidis . Bact. Proc56
    [Google Scholar]
  10. Feldman H. A. 1965; Retaliation by meningococci. Lancet, i436
    [Google Scholar]
  11. Jewell R. P. 1958; Studies to find an inhibitor-free media for sensitivity on the sulfonamides. Am. J. med. Technol 24:371
    [Google Scholar]
  12. Landman O. E., Halle S. 1963; Enzymically and physically induced inheritance changes in Bacillus subtilis . J. molec. Biol 7:721
    [Google Scholar]
  13. Lederberg J., StClair J. 1958; Protoplasts and L-type growth of Escherichia coli . J. Bact 75:143
    [Google Scholar]
  14. Madoff S. 1960; Isolation and identification of PPLO. Ann. N.Y. Acad. Sci 79:383
    [Google Scholar]
  15. Madoff S., Dienes L. 1958; L forms from pneumococci. J. Bact 76:245
    [Google Scholar]
  16. Menzel A. E. O., Rake G. 1942; Studies on meningococcal infection. XII. Immuno-chemical studies on meningococcus type II. J. exp. Med 75:437
    [Google Scholar]
  17. Rotta J., Karakawa W. W., Krause R. M. 1965; Isolation of L forms from group A streptococci exposed to bacitracin. J. Bact 89:1581
    [Google Scholar]
  18. Sharp J. T. 1954; L colonies from hemolytic streptococci: new technic in the study of L forms of bacteria. Proc. Soc. exp. Biol. Med 87:94
    [Google Scholar]
  19. Smith P. F. 1964; Comparative physiology of pleuropneumonia-like and L-type organisms. Bact. Rev 28:97
    [Google Scholar]
  20. Ward J. R., Martin C. H. 1962; Production of L phase variants of bacteria with cycloserine. Proc. Soc. exp. Biol. Med 111:156
    [Google Scholar]
  21. Williams R. E. O. 1963; L forms of Staphylococcus aureus. J. gen. Microbiol 33:325
    [Google Scholar]
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