1887

Abstract

Summary: Oxidation of several substrates by whole or broken organisms of was arrested following treatment with lysozyme, unless an osmotic stabilizer (sorbitol) were present. Whole organisms, which did not oxidize NADH, and broken organisms (which oxidized NADH only slowly) oxidized NADH readily after lysozyme treatment, though the activity was maintained only in presence of an osmotic stabilizer. Lysozyme treatment similarly allowed whole or broken to oxidize (mammalian) cytochrome or to reduce this cytochrome with appropriate electron-donors, e.g. lactate, succinate, or NADH. Most of these observations held good also for the lysozyme-sensitive but not for several lysozyme-insensitive organisms (both Gram-positive and Gram-negative).

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/content/journal/micro/10.1099/00221287-44-1-1
1966-07-01
2021-10-23
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References

  1. Berger L. E., Weiser R. S. 1957; The β-glucosaminidase activity of egg-white lysozyme. Biochim. biophys. Acta 26:517
    [Google Scholar]
  2. Cole H. A., Hughes D. E. 1965; The enzymic activity of the outer shell of Lactobacillus arabinosus . J. gen. Microbiol 40:81
    [Google Scholar]
  3. Hogeboom G. H., Schneider W. C. 1952; Cytochemical studies; physical state of certain respiratory enzymes of mitochondria. J. biol. Chem 194:513
    [Google Scholar]
  4. Horecker B. L., Kornberg A. 1945; Extinction coefficients of the reduced band of pyridine nucleotides. J. biol. Chem 175:385
    [Google Scholar]
  5. Meyer K., Palmer J. W., Thompson R., Khorazo D. 1936; On the mechanism of lysozyme action. J. biol. Chem 113:479
    [Google Scholar]
  6. Pandya K. P. 1963 Quinones and electron transport in bacteria Ph.D. Thesis: Liverpool;
    [Google Scholar]
  7. Salton M. R. J. 1953; The composition of the cell wall of some Gram-positive and Gram-negative bacteria. Biochim. biophys. Acta 10:512
    [Google Scholar]
  8. Salton M. R. J. 1964 The Bacterial Cell Wall Amsterdam: Elsevier Publishing Co.:
    [Google Scholar]
  9. Smith L. 1954; An investigation of cytochrome c oxidase activity in bacteria. Archs Biochem. Biophys 50:315
    [Google Scholar]
  10. Smith L. 1961; Cytochrome systems in aerobic electron transport. The Bacteria Ed. by Gunsalus I. C., Stanier R. Y. 2365 New York: Academic Press;
    [Google Scholar]
  11. Weibull C. 1953; The isolation of protoplasts from Bacillus megaterium by controlled treatment with lysozyme. J. Bact 66:688
    [Google Scholar]
  12. Weibull C. 1956; Bacterial protoplasts; their formation and characteristics. Bacterial Anatomy, Symp. Soc. gen. Microbiol 6:111
    [Google Scholar]
  13. Wiame J. M., Strock R., Vanderwinkel E. 1955; Biosynthése induite d’arabokinase dans les protoplasts de Bacillus subtilis . Biochim. biophys. Acta 18:353
    [Google Scholar]
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