@article{mbs:/content/journal/micro/10.1099/00221287-43-3-439, author = "Keeler, R. F. and Ritchie, A. E. and Bryner, J. H. and Elmore, Jane", title = "The Preparation and Characterization of Cell Walls and the Preparation of Flagella of Vibrio fetus", journal= "Microbiology", year = "1966", volume = "43", number = "3", pages = "439-454", doi = "https://doi.org/10.1099/00221287-43-3-439", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-43-3-439", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY Methods are described for the preparation of cell-wall material and flagella from Vibrio fetus. In common with certain other Gram-negative organisms V. fetus apparently possesses a three-layer wall which, it is proposed, consists of an outer lipoprotein layer, a middle lipo-poly-saccharide layer, and an inner mucopeptide layer. These layers were studied chemically and by electron microscopy after various extraction and enzymic degradation procedures with whole-wall material. None of the layers of the wall was found to possess a microscopically discernible substructure, although the extracted lipopolysaccharide agglomerated into a characteristic stringy array. The probable function, contribution to cellular rigidity, and composition of the wall layers is discussed.", }