1887

Abstract

SUMMARY

Methods are described for the preparation of cell-wall material and flagella from In common with certain other Gram-negative organisms apparently possesses a three-layer wall which, it is proposed, consists of an outer lipoprotein layer, a middle lipo-poly-saccharide layer, and an inner mucopeptide layer. These layers were studied chemically and by electron microscopy after various extraction and enzymic degradation procedures with whole-wall material. None of the layers of the wall was found to possess a microscopically discernible substructure, although the extracted lipopolysaccharide agglomerated into a characteristic stringy array. The probable function, contribution to cellular rigidity, and composition of the wall layers is discussed.

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/content/journal/micro/10.1099/00221287-43-3-439
1966-06-01
2022-01-20
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