SUMMARY: Methods are described for the preparation of cell-wall material and flagella from In common with certain other Gram-negative organisms apparently possesses a three-layer wall which, it is proposed, consists of an outer lipoprotein layer, a middle lipo-poly-saccharide layer, and an inner mucopeptide layer. These layers were studied chemically and by electron microscopy after various extraction and enzymic degradation procedures with whole-wall material. None of the layers of the wall was found to possess a microscopically discernible substructure, although the extracted lipopolysaccharide agglomerated into a characteristic stringy array. The probable function, contribution to cellular rigidity, and composition of the wall layers is discussed.


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