@article{mbs:/content/journal/micro/10.1099/00221287-43-3-427, author = "Ritchie, A. E. and Keeler, R. F. and Bryner, J. H.", title = "Anatomical Features of Vibrio fetus: Electron Microscopic Survey", journal= "Microbiology", year = "1966", volume = "43", number = "3", pages = "427-438", doi = "https://doi.org/10.1099/00221287-43-3-427", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-43-3-427", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "SUMMARY Vibrio fetus was examined in shadow-cast, negative-contrast and thin-sectioned preparations; Four morphological types were noted in shadowed specimens: comma, coccoid, S-shaped and filamentous. In negative-contrast preparations organisms were differentiated into two types on the appearance of their cytoplasmic mass. A large spherical cytoplasmic inclusion was frequently evident at the flagellated pole of freshly harvested organisms. Various morphological variants, ‘sports’, e.g. multiflagellate, were easily detected. A non-motile isolate was either devoid of flagella or exhibited only a short stub at the pole. In thin-section preparations anatomical features noted were: a rugose loosely fitting membranous integument; a complex cytoplasmic membrane of honeycomb-like structure; a fibrillar nucleoplasm and fibrillar cytoplasmic ground substance with associated ribosomes; lamellated or cytoplasmic granules bounded by a smooth membrane. At the flagellated pole the cytoplasmic ground substance was generally rarefied and a cone-shaped flagellar basal granule was present. The flagella were enlarged at their proximal terminus. Mesosome-like elaborations of the cytoplasmic membrane were not observed. It was concluded that Vibrio fetus closely resembles members of the genus Spirillum. ", }