1887

Abstract

SUMMARY

Serologically active fractions from 8 strains of , each with a different type antigen, were obtained by partial purification on a DEAE-cellulose column. By qualitative analysis of the hydrolysates of these fractions it was shown that glucose and glucosamine were always present, galactosamine and galactose were generally present. Arabinose was present as a minor component in some cases, whereas rhamnose was never found. Some evidence suggested that the glucose present in these hydrolysates was at least partly derived from the group D teichoic acid. -acetylglucosamine or -acetylgalactosamine and their corresponding hexoses were the main inhibitors of the quantitative precipitin reaction in 4 out of 8 type antigen systems investigated. In the type 19 system the two -acetylhexosamines but no other sugars were inhibitory. No inhibition by simple sugars was observed with the remaining three systems. The conditions required to obtain serologically active extracts are discussed. Two kinds of inhibitors formed during HCl extraction were isolated.

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/content/journal/micro/10.1099/00221287-43-3-375
1966-06-01
2021-10-16
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References

  1. Bleiweis A. S., Krause R. M. 1965; The cell walls of group D streptococci. I. The immunochemistry of the type 1 carbohydrate. J. exp. Med 122:237
    [Google Scholar]
  2. Cummins C. S., Harris H. 1956; The chemical composition of the cell wall in some Gram-positive bacteria and its possible value as a taxonomic character. J. gen. Microbiol 14:583
    [Google Scholar]
  3. Curtis S. N., Krause R. M. 1964; Immunochemical studies on the cell walls of group G haemolytic streptococci. Fed. Proc 22:205
    [Google Scholar]
  4. Elliott S. D. 1960; Type and group polysaccharides of group D streptococci. J. exp. Med 111:621
    [Google Scholar]
  5. Jones D., Shattock P. M. F. 1960; The location of the group antigen of group D streptococcus. J. gen. Microbiol 23:335
    [Google Scholar]
  6. Krause R. M., McCarty M. 1962; Studies on the chemical structure of the streptococcal cell wall. II. The composition of group C cell walls and chemical basis for serologic specificity of the carbohydrate moiety. J. exp. Med 115:49
    [Google Scholar]
  7. McCarty M. 1952; The lysis of group A hemolytic streptococci by extracellular enzymes of Streptomyces albus. I. Production and fractionation of the lytic enzymes. J. exp. Med 96:555
    [Google Scholar]
  8. McCarty M. 1958; Further studies on the chemical basis for serological specificity of group A streptococcal carbohydrate. J. exp. Med 108:311
    [Google Scholar]
  9. Maxted W. R., Fraser C. A. M. 1964; The serological typing of Streptococcus faecalis. Zentbl. Bakt.ParasitKde Abt. 2 196:76
    [Google Scholar]
  10. Maxted W. R., Fraser C. A. M. 1966; Sensitivity to acid of the type antigens of Streptococcus faecalis. J. gen. Microbiol 43:145
    [Google Scholar]
  11. Sharpe M. E. 1964; Serological types of Streptococcus faecalis and its varieties and their cell wall type antigen. J. gen. Microbiol 36:151
    [Google Scholar]
  12. Sharpe M. E., Fewins B. G. 1960; Serological typing of strains of Streptococcus faecium and unclassified group D streptococci isolated from canned hams and pig intestines. J. gen. Microbiol 23:621
    [Google Scholar]
  13. Sharpe M. E., Shattock P. M. F. 1952; The serological typing of group d streptococci associated with outbreaks of neonatal diarrhoea. J. gen. Microbiol 6:150
    [Google Scholar]
  14. Slade H. D., Slamp W. C. 1962; Cell-wall composition and the grouping antigens of streptococci. J. Bact 84:345
    [Google Scholar]
  15. Uchida T., Robbins P. W., Luria S. E. 1963; Analysis of the serologic determinant groups of the Salmonella E group O-antigens. Biochemistry 2:663
    [Google Scholar]
  16. Wicken A. J., Elliott S. D., Baddiley J. 1963; The identity of streptococcal group d antigen with teichoic acid. J. gen. Microbiol 31:231
    [Google Scholar]
  17. Willers J. M. N., Michel M. F., Sysma M. J., Winkler K. C. 1964; Chemical analysis and inhibition reactions of the group and type antigens of group F streptococci. J. gen. Microbiol 36:95
    [Google Scholar]
  18. Wittner M. K., Hayashi J. A. 1965; Studies of streptococcal cell walls. VII. Carbohydrate composition of group B cell walls. J. Bact 89:398
    [Google Scholar]
  19. Yamakawa T., Nishimura S., Kamimura M. 1965; The chemistry of the lipids of posthemolytic residue or stroma of erythrocytes. XIII. Further studies on human red cell glycolipids. Jap. J. exp. Med 35:201
    [Google Scholar]
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