1887

Abstract

SUMMARY

organisms of a very young culture released neurotoxin into the medium within 1 hr after it was infected with T-phages. Two types of phage-associated enzymes were considered to be involved in this process. The first type was phage-bound enzyme, which was bound firmly with phage, worked together with phage, and depolymerized neurotoxin into smaller molecules. The second type enzyme was phage-induced lysin, so-called ‘endolysin’, which was separable from phage and had lytic activity on dead bacteria. The latter enzyme, however, had no direct effect on the amount of neurotoxin released from acetone-powder preparations of the bacteria into phosphate buffer, although endolysin lysed 25 % of the bacteria at 30° in 30 min. Young living organisms did not release neurotoxin into the medium under normal conditions, whereas the acetone-powder preparations of , i.e. dead organisms, released neurotoxin into the medium freely under the same conditions.

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/content/journal/micro/10.1099/00221287-43-1-83
1966-04-01
2022-01-17
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References

  1. Adams M. H. 1959 Bacteriophages New York: Academic Press;
    [Google Scholar]
  2. Barksdale W. L., Pappenheimer A. M. 1954; Phage-host relationships in non-toxigenic and toxigenic diphtheria bacilli. J. Bact 67:220
    [Google Scholar]
  3. Boivin A. 1940; Les deux toxines du bacille de Shiga et leur place dans la classification générale des toxines bactériennes. Revue Immunol. Thér. antimicrob 6:86
    [Google Scholar]
  4. Dubos R., Geiger J. W. 1946; Preparation and properties of Shiga toxin and toxoid. J. exp. Med 84:143
    [Google Scholar]
  5. Engley F. B. Jun 1952; The neurotoxin of Shigella dysenteriae. Bact. Rev 16:153
    [Google Scholar]
  6. Li K., Barksdale W. L., Garmise L. 1961; Phenotypic alterations associated with bacteriophage carrier state of Shigella dysenteriae. J. gen. Microbiol 24:355
    [Google Scholar]
  7. Mueller J. H., Miller P. A. 1941; Production of diphtheric toxin of high potency (100 Lf) on a reproducible medium. J. Immun 40:21
    [Google Scholar]
  8. Šourek J., Raška K. Jr 1963; A contribution to the study of Shigella dysenteriae toxins. J. Hyg. Epidem. Microbiol. Immun 7:46
    [Google Scholar]
  9. Van Heyningen W. E., Gladstone G. P. 1953; The neurotoxin of Shigella shigae. 1. Production, purification and properties of the toxin. Br. J. exp. Path 34:202
    [Google Scholar]
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