1887

Abstract

SUMMARY

A plaque assay method is described for the titration of the infective ribonucleic acid (RNA) of foot-and-mouth disease (FMD)> virus. The method depends on the dilution of the RNA in salt solutions of high molarity and the treatment of the BHK 21 cell monolayers used with M-NaCl. The method is reproducible and is at least as sensitive as other methods of titration. The extraction of RNA in the presence of EDTA gave titres corresponding to 0.1 % of the titre of the intact virus. RNA prepared from virus suspensions containing EDTA retained about 50% of initial infectivity for 7 days at 4° but for longer periods it was best preserved in 70 % (v/v) ethanol at 20°.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-43-1-59
1966-04-01
2021-10-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/43/1/mic-43-1-59.html?itemId=/content/journal/micro/10.1099/00221287-43-1-59&mimeType=html&fmt=ahah

References

  1. Alexander H. E., Koch G., Mountain I. M., van Damme O. 1958; Infectivity of ribonucleic acid from poliovirus in human cell monolayers. J. exp. Med 108:493
    [Google Scholar]
  2. Bachrach H. L. 1960; Ribonucleic acid of foot-and-mouth disease virus: its preparation, stability and plating efficiency on bovine-kidney cultures. Virology 12:258
    [Google Scholar]
  3. Brown F., Cartwright B. 1963; Purification of radioactive foot-and-mouth disease virus. Nature, Land 199:1168
    [Google Scholar]
  4. Brown F., Sellers R. F., Stewart D. L. 1958; Infectivity of ribonucleic acid from mice and tissue culture infected with the virus of foot-and-mouth disease. Nature, Lond 182:535
    [Google Scholar]
  5. Brown F., Stewart D. L. 1959; Studies with infective ribonucleic acid from tissues and cell cultures infected with the virus of foot-and-mouth disease. Virology 7:408
    [Google Scholar]
  6. Colon J. I., Idoine J. B. 1964; Factors affecting plaque formation by the infectious ribonucleic acid of the equine encephalitis viruses. J. infect. Dis 114:61
    [Google Scholar]
  7. Ellem K. A. O., Colter J. S. 1961; The interaction of infectious ribonucleic acids with mammalian cells. III. Comparison of infection and RNA uptake in the HeLa cell-polio RNA and L. cell-Mengo RNA systems. Virology 15:113
    [Google Scholar]
  8. Fraenkel-Conrat H. 1957 The infectivity of tobacco mosaic virus nucleic acid. Cellular Biology, Nucleic Acids and Viruses217 New York: New York Academy of Sciences;
    [Google Scholar]
  9. Fraenkel-Conrat H., Singer B., Tsugita A. 1961; Purification of viral RNA by means of bentonite. Virology 14:54
    [Google Scholar]
  10. Franklin R. M., Wecker E., Henry C. 1959; Some properties of an infectious ribonucleic acid from mouse encephalomyelitis virus. Virology 7:220
    [Google Scholar]
  11. Hantschel H. 1963; Infektiöse Ribonukleinsäure aus neurotropem modifiziertem MKS-Typ A. Arch. exp. Vet. Med 17:263
    [Google Scholar]
  12. Hantschel H., Veckenstedt A. 1964; Infektiöse Ribonukleinsäure aus neurotropen Varianten des Maul-und-Klauenseuche-Virus. Arch. exp. Vet. Med 18:409
    [Google Scholar]
  13. Holland J. T., Hoyer B. H., McLaren L. C., Syverton J. T. 1960; Enteroviral ribonucleic acid. I. Recovery from virus and assimilation by cells. J. exp. Med 112:821
    [Google Scholar]
  14. Koch G., Koenig S., Alexander H. E. 1960; Quantitative studies on the infectivity of ribonucleic acid from partially purified and highly purified poliovirus preparations. Virology 10:329
    [Google Scholar]
  15. Macpherson I. A., Stoker M. G. P. 1962; Polyoma transformation of hamster cell clones—an investigation of genetic factors affecting cell competence. Virology 16:147
    [Google Scholar]
  16. Montagnier L., Sanders F. K. 1962; Titrage de l’acide ribonucléique infectieux du virus de l’encéphalomyocardite de la souris sur cellules d’ascite Krebs II. in vitro. C. r. hebd. Séanc. Acad. Sci., Paris 254:2247
    [Google Scholar]
  17. Mowat G. N., Chapman W. G. 1962; Growth of foot-and-mouth disease virus in a fibroblastic cell line derived from hamster kidneys. Nature, Lond 194:253
    [Google Scholar]
  18. Mussgay M., Strohmaier K. 1958; Gewinnung eines infektiösen Prinzips von Ribonukleinsäure-Charakter aus Homogenaten mit dem Maul-und-Klauenseuche-Virus infizierter Jungmäuse. Zentbl. Bakt.ParasitKde 1. Abt. Orig. 173:163
    [Google Scholar]
  19. Norman A., Veomett R. C. 1961; Ribonuclease activity at the HeLa cell surface. Virology 14:497
    [Google Scholar]
  20. Reed L. T., Muench H. 1938; A simple method for estimating 50 per cent endpoints. Am. J. Hyg 27:493
    [Google Scholar]
  21. Skinner H. H. 1951; Propagation of strains of foot-and-mouth disease virus in unweaned white mice. Proc. B. Soc. Med 44:1041
    [Google Scholar]
  22. Sprunt K., Koenig S., Alexander H. E. 1961; Factors influencing the infectivity of poliovirus ribonucleic acid. Proc. Soc. exp. Biol. Med 108:755
    [Google Scholar]
  23. Thely M., Sach E., Dhennin Leone, Choay J., Dhennin Louis. 1961; Préparation à l’aide de la bentonite d’un acide ribonucléique hautement infectieux extrait d’epi-thélium lingual de bovins atteints de fièvre aphteuse. C. r. hebd. Séanc. Acad. Sci., Paris 253:3118
    [Google Scholar]
  24. Thomas J. A., Leclerc J. 1959; L’acide ribonucléique infectieux du virus de la fièvre aphteuse. C. r. hebd. Séanc. Acad. Sci., Paris 248:606
    [Google Scholar]
  25. Thomas J. A., Leclerc J. 1960; Sur le pouvoir infectieux et sur quelques propriétés de l’acide ribonucléique extrait du virus de la fièvre aphteuse. Bull. Off. int. Epiz 53:1328
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-43-1-59
Loading
/content/journal/micro/10.1099/00221287-43-1-59
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error