1887

Abstract

SUMMARY

A chemically defined culture medium was used to study the effect of Parified lactoperoxidase and thiocyanate on the growth of several cultures of and While not inhibited by either component alone, growth was completely inhibited when both components were present in the medium. The growth inhibition was annulled completely by glutathione, thioglycollic (mercaptoacetic) acid of catalase. glyceraldehyde phosphate dehydrogenase was inhibited by lactoperoxidase when hydrogen peroxide was present. The inhibition was annulled with cysteine and glutathione which suggested this dehydrogenase to be a possible site of inhibition. The inhibition was Postulated to be a peroxidatic conversion of essential enzymic sulphydryl groups to inactive disulphide groups, thus interfering with the energy metabolism up of With cultures a delay in growth inhibition up to 6 hr resulted instead of complete growth inhibition. Catalase neutralized this effect. The extent of growth inhibition was greatest in those strains which were unable to adapt to an oxidative pathway for their energy supply. In becoming independent of the fermentative pathway, the cultures were no longer as sensitive to peroxidase, thioeysnate and hydrogen peroxide. The necessity for thiocyanate in the is inhibitory system is not yet clear. Thiouracil and thiourea were ineffective replacements for thiocyanate.

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/content/journal/micro/10.1099/00221287-43-1-31
1966-04-01
2022-01-19
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References

  1. Auclair J. E. 1954; The inhibition of microorganisms by raw milk. III. Distribution and properties of two inhibitory substances, lactenin 1 and lactenin 2. J. Dairy Res 21:323
    [Google Scholar]
  2. Auclair J. E., Hirsch A. 1953; The inhibition of microorganisms by raw milk. I. The occurrence of inhibitory and stimulatory phenomena. Methods of estimation. J. Dairy Res 20:45
    [Google Scholar]
  3. Colowick S., Kaplan N. O. 1959 Methods in Enzymology i:401
    [Google Scholar]
  4. DeLuca C, Weber M. M., Kaplan N. O. 1956; A specific spectrophotometry assay for flavinadeninedinucleotide. J. biol. Chem 223:559
    [Google Scholar]
  5. Dixon M., Webb E. C. 1958 Enzymes409 New York: Academic Press, Inc;
    [Google Scholar]
  6. Greisen E. C., Gunsalus I. C. 1944; An alcohol oxidation system in a streptococcus which functions without hydrogen peroxide accumulation. J. Bact 48:515
    [Google Scholar]
  7. Jago G. R., Morrison M. 1962; Antistreptococcal activity of lactoperoxidase. III. Proc. Soc. exp. Biol. Med Ill:585
    [Google Scholar]
  8. Jones F. S., Simms H. F. 1930; The bacterial growth inhibitor (lactenin) of milk. I. The preparation in concentrated form. J. exp. Med 51:327
    [Google Scholar]
  9. Krimsky I., Racker E. 1952; Glutathione: a prosthetic group of triosephosphate dehydrogenase. J. biol. Chem 198:721
    [Google Scholar]
  10. Mickelson M. N. 1964; Chemically defined medium for growth of Streptococcus pyogenes. J. Bact 88:158
    [Google Scholar]
  11. Morrison M., Hultquist D. E. 1963; Lactoperoxidase. II. Isolation. J. biol. Chem 238:2847
    [Google Scholar]
  12. Portmann A., Auclair J. E. 1959; Relation entre la lactenin L2 et la lactoperoxydase. Le Laü 39:147
    [Google Scholar]
  13. Reiter S., Pickering A., Oram J. D. 1964 An inhibitory system: lactoperoxidase/ thiocyanate/hydrogen peroxide in raw milk, éth int. Symp. Food Microbiology297 SIK; Goteberg, Sweden:
    [Google Scholar]
  14. Reiter B., Pickering A., Oram J. D., Pope G. S. 1963; Peroxide, thiocyanate inhibition of streptococci in raw milk. J. gen. Microbiol 33:xii
    [Google Scholar]
  15. Seeley H. W., Vandemark P. J. 1951; An adaptive peroxidation by S. faecalis. J. Bact 61:27
    [Google Scholar]
  16. Sörbo H., Ljunggren J. G. 1958; The catalytic effect of peroxidase on the reaction of hydrogen peroxide and certain sulfur compounds. Acta chem. scand 12:470
    [Google Scholar]
  17. Stadtman E. R., Novelli G. D., Lipmann F. 1951; Coenzyme A function in acetyl transfer by a phosphotransacetylase system. J. biol. Chem 191:865
    [Google Scholar]
  18. Umbreit W. W., Burris R. H., Stauffer J. F. 1957 Manometric techniques, 2nd ed.. Minneapolis, Minn.: Burgess Publishing Co;
    [Google Scholar]
  19. Wilson A. T., Rosenblum H. 1952; The antistreptococcal property of milk. I. Some characteristics of the activity of lactenin in vitro. The effect of lactenin on hemolytic streptococci of several serological groups. J. exp. Med 95:25
    [Google Scholar]
  20. Wilson A. T., Rosenblum H. 1952; The antistreptococcal property of milk. II. The effects of anaerobiosis, reducing agents, thiamine and other chemicals on lactenin action. J. exp. Med 95:39
    [Google Scholar]
  21. Wright R. C., Tramer J. 1958; Factors affecting the activity of cheese starters. The role of milk peroxidase. J. Dairy Bes 25:104
    [Google Scholar]
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