SUMMARY: The resistance of 148 clinical isolates of Gram-negative bacteria to ampicillin and benzylpenicillin was determined in serial-dilution sensitivity tests, together with the extent of inactivation of the penicillins, and the production of 6-aminopenicillanic acid (6-). Many of the cultures were resistant to the penicillins and inactivated the compounds, but only one culture, a strain of showed penicillin acylase activity, as indicated by the production of 6- However, the cultural conditions prevailing in serial-dilution tests, namely, stationary culture at 37°, although favourable for the functioning of penicillin acylases, were shown to be highly unfavourable for production of the enzyme by coliform bacteria.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error