1887

Abstract

SUMMARY: The intracellular concentrations of C-streptomycin accumulated by strain B during growth in the presence of different extracellular concentrations of the compound have been measured. They increased logarithmically with time at rates proportional to the logarithm of the extracellular concentration; the growth rates declined linearly with time at rates which were also proportional to the extracellular concentrations. Thus the same decrease in growth rate resulted from different intracellular concentrations of streptomycin, according to the conditions of uptake. After removal of extracellular streptomycin, the growth rates remained constant for several hours, during which time 40-60% of the total cell-bound radioactivity was lost. Of the radioactivity lost from the organisms during growth after removal of extracellular streptomycin, 50-80% was recovered from culture filtrates but did not behave as streptomycin with respect to adsorption to charcoal.

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/content/journal/micro/10.1099/00221287-42-3-333
1966-03-01
2021-10-23
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References

  1. Cox E. C, White J. R., Flaks J. G. 1964; Streptomycin action and the ribosome. Proc. natn. Acad. Sci. U.S.A 51:703
    [Google Scholar]
  2. Davies J. E. 1964; Studies on the ribosomes of streptomycin-sensitive and resistant strains of Escherichia coli. Proc. natn. Acad. Sci. U.S.A 51:659
    [Google Scholar]
  3. Dubin D. T., Hancock R., Davis B. D. 1963; The sequence of some effects of streptomycin in Escherichia coli. Biochim. Biophys. Acta 74:476
    [Google Scholar]
  4. Erdös T., Ullmann A. 1959; Effect of streptomycin on the incorporation of amino-acids labelled with carbon-14 into ribonucleic acid and protein in a cell-free system of a Mycobacterium. Nature, Lond 183:618
    [Google Scholar]
  5. Flaks J. G., Cox E. C., White J. R. 1962; Inhibition of polypeptide synthesis by streptomycin. Biochem. Biophys. Res. Commun 7:385
    [Google Scholar]
  6. Hahn F. E., Ciak J., Wolfe A. D., Hartman R. E., Allison J. L., Hartman R. S. 1962; Studies on the mode of action of streptomycin II. Effects of streptomycin on the synthesis of proteins and nucleic acids and on cellular multiplication in Escherichia coli. Biochim. Biophys. Acta 61:741
    [Google Scholar]
  7. Hancock R. 1962a; Uptake of 14C-streptomycin by some micro-organisms and its relation to their streptomycin sensitivity. J. gen. Microbiol 28:493
    [Google Scholar]
  8. Hancock R. 1962b; Uptake of streptomycin by Bacillus megaterium. J. gen. Microbiol 28:503
    [Google Scholar]
  9. Humphrey J. H., Lightbown J. W. 1952; A general theory for plate assay of antibiotics with some practical applications. J. gen. Microbiol 7:129
    [Google Scholar]
  10. Humphrey J. H., Lightbown J. W., Mussett M. V. 1959; The second international standard for streptomycin. Bull. Wld Hlth Org 20:1233
    [Google Scholar]
  11. Hurwitz C., Rosano C. L. 1962; Accumulation of label from 14C-streptomycin by Escherichia coli. J. Bact 83:1193
    [Google Scholar]
  12. Kjeldgaard N. O., Kurland C. G. 1963; The distribution of soluble and ribosomal RNA as a function of growth rate. J. molec. Biol 6:341
    [Google Scholar]
  13. Kogut M., Lightbown J. W. 1964a; The mode of action of streptomycin. The effects of intracellular antibiotic on the growth of Escherichia coli. Antibiotica Congressus Praha. Abstr. A1-3:5
    [Google Scholar]
  14. Kogut M., Lightbown J. W. 1964b The mode of action of streptomycin. Experimental Chemotherapy Schnitzer R. J., Hawking F. 339 New York: Academic Press;
    [Google Scholar]
  15. Kogut M., Lightbown J. W., Isaacson P. 1965a; Streptomycin action and anaero-biosis. J. gen. Microbiol 39:155
    [Google Scholar]
  16. Kogut M., Lightbown J. W., Isaacson P. 1965b; Effects of dihydrostreptomycin treatment on the growth of Esherichia coli after removal of extracellular antibiotic. J. gen. Microbiol 39:165
    [Google Scholar]
  17. Litwak G., Pramer D. 1957; Absorption of antibiotics by plant cells. III. Kinetics of streptomycin uptake. Arch. Biochem. Biophys 68:396
    [Google Scholar]
  18. Plotz P. H., Dubin D. T., Davis B. D. 1961; Influence of salts on the uptake of streptomycin by Escherichia coli. Nature, Lond 191:1324
    [Google Scholar]
  19. Pramer D. 1956; Absorption of antibiotics by plant cells. II. Streptomycin. Arch. Biochem. Biophys 62:265
    [Google Scholar]
  20. Rossett R., Monier R., Julien J. 1964; RNA composition of Escherichia coli as a function of growth rate. Biochem. Biophys. Res. Commun 15:329
    [Google Scholar]
  21. Spotts C. R., Stanier R. Y. 1961; Mechanism of streptomycin action on bacteria: a unitary hypothesis. Nature, Lond 192:638
    [Google Scholar]
  22. Szybalski W., Mashima S. 1959; Uptake of streptomycin by sensitive, resistant and dependent bacteria. Biochem. Biophys. Res. Commun 1:249
    [Google Scholar]
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