1887

Abstract

SUMMARY: Streptogramin is a complex antibiotic containing two components, streptogramin A and streptogramin B. The individual components are bacteriostatic whereas the mixture is bactericidal. At growth-limiting concentrations streptogramin A and the streptogramin complex inhibit by blocking protein synthesis but not nucleic acid and cell wall synthesis. Protein, nucleic acid and cell wall synthesis were all reduced in the presence of streptogramin B.

Streptogramin A, but not streptogramin B, inhibits the binding of radioactive chloramphenicol to bacterial ribosomes. However, streptogramin B enhances this effect of streptogramin A in a cell-free system containing both bacterial soluble fraction and ribosomes. The results suggest that streptogramin A inhibits protein synthesis by bacteria at the ribosome level, and that this effect is enhanced by streptogramin B.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-42-1-93
1966-01-01
2024-11-06
Loading full text...

Full text loading...

References

  1. Anand N., Davis B. D. 1960; Effect of streptomycin on Escherichia coli. Damage by streptomycin to the cell membrane of E. coli. Nature, Lond 185:22
    [Google Scholar]
  2. Arai M., Nakamura S., Sakagami Y., Fukuhara K., Yonehara H. 1956; A new antibiotic, mikamycin. J. Antibiot. (Japan), Ser. A 9:193
    [Google Scholar]
  3. Ball S., Boothroyd B., Lees K. A., Raper A. H., Lester Smith E. 1958; Preparation and properties of an antibiotic complex E 129. Biochem. J 68:24
    [Google Scholar]
  4. Bodanszky M., Ondetti A. 1963; Structures of the vernamycin B group of antibiotics. Antimicrobial Ag. & Chemother360
    [Google Scholar]
  5. Benazet F., Cosar C, Dubost M., Julou L., Mancy D. 1962; Un nouvel antibiotique, la pristinamycine (7293 R.P.). Sem. Hop. Paris 38:13
    [Google Scholar]
  6. Burton K. 1956; A study of the conditions and mechanisms of the diphenylamine estimation of desoxyribonucleic acid. Biochem. J 62:315
    [Google Scholar]
  7. Celmer W. D., Sobin B. A. 1955/56; The isolation of two synergistic antibiotics from a single fermentation source. Antibiotics Ann437
    [Google Scholar]
  8. Charney J., Fisher W. P., Curran C, Machlowitz R. A., Tytell A. A. 1953; Streptogramin, a new antibiotic. Antiobiotics & Chemother 3:1283
    [Google Scholar]
  9. Coleman V. R., Gunnison J. B., Ja wetz E. 1953; Participation of erythromycin and carbomycin in combined antibiotic action in vitro. Proc. Soc. exp. Biol. Med 83:668
    [Google Scholar]
  10. de Somer P., van Duck P. 1955; A preliminary report on antibiotic number 899, a streptogramin-like substance. Antibiotics & Chemother 5:632
    [Google Scholar]
  11. Gale E. F. 1947; The assimilation of amino acids by bacteria. 1. The passage of certain amino acids across the cell wall and their concentration in the internal environment of Streptococcus faecalis. J. gen. Microbiol 1:53
    [Google Scholar]
  12. Gale E. F. 1963; Mechanisms of antibiotic action. Pharmacol. Rev 15:481
    [Google Scholar]
  13. Gale E. F., Folkes J. P. 1953a; The assimilation of amino acids by bacteria. 14. Nucleic acid and protein synthesis in Staphylococcus aureus. Biochem. J 53:483
    [Google Scholar]
  14. Gale E. F., Folkes J. P. 1953b; The assimilation of amino acids by bacteria. 15. Actions of antibiotics on nucleic acid and protein synthesis in Staphylococcus aureus. Biochem. J 53:493
    [Google Scholar]
  15. Garcia-Mendoza C. 1965; Studies on the mode of action of etamycin (viridogrisein). Biochim. biophys. Acta 97:394
    [Google Scholar]
  16. Garrod L. P., Waterworth P. M. 1956; Behaviour in vitro of some new anti-staphylococcal antibiotics. Brit. med. J ii:61
    [Google Scholar]
  17. Hancock R. 1960; Accumulation of pool amino acids in Staphylococcus aureus following inhibition of protein synthesis. Biochim. biophys. Acta 37:47
    [Google Scholar]
  18. Hancock R., Park J. T. 1958; Cell wall synthesis by Staphylococcus aureus in the presence of chloramphenicol. Nature, Lond 181:1050
    [Google Scholar]
  19. Hurwitz C., Rosano C. L. 1962; Chloramphenicol sensitive and insensitive phases of the lethal action of streptomycin. J. Bact 83:1202
    [Google Scholar]
  20. Jawetz E., Gunnison J. B., Bruff J. B., Coleman V. R. 1952; Studies on antibiotic synergism and antagonism. J. Bact 64:29
    [Google Scholar]
  21. Kirby W. M. M., Burnell J. M. 1954; Effect of combinations of antibiotics on lysis of Staphylococcus aureus by penicillin. J. Bact 67:50
    [Google Scholar]
  22. Laskin A. I., May Chan W. 1964; Inhibition by vernamycin A of amino acid incorporation in Escherichia coli cell-free systems. Antimicrobial Ag. & Chemother485
    [Google Scholar]
  23. Lester Smith E. 1963; The ostreogrycins. A family of synergistic antibiotics. Proc. Soc. gen. Microbiol 33:iii
    [Google Scholar]
  24. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin-phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  25. McQuillen K., Roberts R. B. 1954; The utilization of acetate for synthesis in Escherichia coli. J. biol. Chem 207:81
    [Google Scholar]
  26. Miles A. A., Misra S. S. 1938; The estimation of the bactericidal power of the blood. J. Hyg., Camb 38:732
    [Google Scholar]
  27. Prestidge L. S., Pardee A. B. 1957; Induction of bacterial lysis by penicillin. J. Bact 74:48
    [Google Scholar]
  28. Sarin P. S. 1962; Structural studies on the antibiotics ostreogrycin A and G. Ph.D. Thesis. Cambridge University;
    [Google Scholar]
  29. Umbreit W. W., Burris R. H., Stauffer J. F. 1949 Manometric Techniques and Tissue Metabolism Minneapolis: U.S.A. Burgess Publishing Co;
    [Google Scholar]
  30. Vanderhaeghe H., Van Duck P., Parmentier G., De Somer P. 1957; Isolation and properties of the components of staphylomycin. Antibiotics & Chemother. 7 606:
    [Google Scholar]
  31. Vazquez D. 1962; Studies on the mode of action of streptogramin. Biochim. biophys. Acta 61:849
    [Google Scholar]
  32. Vazquez D. 1963a; The effect of streptogramin and related compounds on Staphylococcus aureus. Proc. Soc. gen. Microbiol 33:ix
    [Google Scholar]
  33. Vazquez D. 1963b; Antibiotics which affect protein synthesis: the uptake of 14C-chloramphenicol by bacteria. Biochem. biophys. Res. Commun 12:409
    [Google Scholar]
  34. Vazquez D. 1964a; The effect of streptogramin A and B on the binding of 14C-labelled chloramphenicol to ribosomes. Proc. Soc. gen. Microbiol 35:v
    [Google Scholar]
  35. Vazquez D. 1964b; The binding of chloramphenicol by ribosomes from Bacillus mega-terium. Biochem. biophys. Res. Commun 15:464
    [Google Scholar]
  36. Vazquez D. 1965a; A method for the preparation of bacterial 50 S ribosomal subunits. Biochim. biophys. Acta 108:154
    [Google Scholar]
  37. Vazquez D. 1965b; Binding of chloramphenicol to ribosomes. The effect of a number of antibiotics. Biochim. biophys. Acta in the Press
    [Google Scholar]
  38. Vazquez D. 1965c; Antibiotics affecting chloramphenicol uptake by bacteria. Their effect on amino acid incorporation in a cell-free system. Biochim. biophys. Acta in the Press
    [Google Scholar]
  39. Yamaguchi H., Tanaka N. 1964; Selective toxicity of mikamycins inhibitors of protein synthesis. Nature, Lond 201:409
    [Google Scholar]
/content/journal/micro/10.1099/00221287-42-1-93
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error