By gel-diffusion precipitin and antigen-absorption tests, 34 strains of PPLO (Mycoplasma hominis, types 1-4) were examined, and 15 antigenic components recognized. Antigens a, b, f and g were common to types 1, 2 and 4; c was specific to type 1; h was specific to type 2. Type 3 was antigenically unrelated to the remainder. Its two recognizable components were named j and k. Three further antigens in type 1 were recognized as 17, 39 and 40, previously defined in strains of Haemophilus and Coryne-bacterium associated with mycoplasma-like variants (Pease & Laughton, 1965); antigen 40 also occurred in type 2. Two diffusible antigens, α and β, were detected in strains of types 1 and 4; these and also g were found to be shared with the bacterial strains.
All genital strains of mycoplasma isolated in this laboratory belonged to type 1, all oral strains to type 4; one rat strain belonged to type 2.
Distribution of antigenic components in strains of mycoplasma and in different strains of bacteria, including those believed to be derived from mycoplasma (or vice versa), and also in others, notably Haemophilus influenzae, supports previous claims of relationship between Mycoplasma hominis types 1, 2 and 4, Corynebacterium and Haemophilus. The validity of Mycoplasma as a distinct group of micro-organisms is thus called into question.
BrownT. McP., NunemakerJ. C.1942; Rat-bite fever. A review of American cases with re-evaluation of etiology: report of cases. Bull. Johns Hopkins Hosp. 70:201
FreimerE. H., KrauseR. M., McCartyM.1959; Studies of l-forms and protoplasts of group A streptococci. I. Isolation, growth and bacteriologie characteristics. J. exp. Med. 110:853
Huijsmans-EversA. G. M., RuysA. C.1956; Microorganisms of the pleuropneumonia group (family of Mycoplasmataceae) in man. II. Serological identification and discussion of pathogenicity. Antonie van Leeuwenhoek 22:377
MinckR.1953; Récherches sur l’origine des organismes du type de la péripneumonie trouvées dans les organes génitaux de la femme. C. r. hebd. Séanc. Acad. Sci., Paris 236:250
PeaseP. E.1962; Evidence that Streptobacillus moniliformis is an intermediate stage between a corynebacterium and its l-form or derived PPLO. J. gen. Microbiol. 29:91
PeaseP. E., BissetK. A.1962; The effect of parasitism on Gram-positive bacteria with reference to the origin of certain types of virus. Nature, Lond. 196:357
PeaseP. E., LaughtonN.1965; The antigenic structure of Haemophilus and Corynebacterium species from the human genital tract claimed to be associated with or derived from Mycoplasma hominis. J. gen. Microbiol. 41:293
Taylor-RobinsonD., SomersonN. L., TurnerH. C., ChanockR. M.1963; Serological relationships among human mycoplasmas as shown by complement fixation and gel diffusion. J. Bact. 85:1261
VillemotJ. M., ProvostA.1959; Récherches immunologiques sur la péripneumonie. VI. Bases d’une classification sérologique des microorganismes du genre Mycoplasma. Revue Élev. Méd. vér. Pays trop. 12:369
WittlerR. G., CaryS. G., LindbergR. B.1956; Reversion of a pleuropneumonia-like organism to a corynebacterium during tissue culture passage. J. gen. Microbiol. 14:763