The Influence of Temperature and pH Value on the Macro-molecular Composition of Magnesium-limited and Glycerol-limited Aerobacter aerogenes Growing in a Chemostat
Progressive alteration of the incubation temperature of Aerobacter aerogenes cultures, growing in a chemostat at a fixed dilution rate, caused a progressive change in bacterial concentration and in bacterial RNA, carbohydrate and protein contents; the DNA content did not vary significantly. The change in bacterial RNA concentration was associated with a variation in bacterial ribosome content, similar to that found in organisms grown at a fixed temperature but different dilution rates. Changes in bacterial yield also resulted from alterations in the pH value of the culture, but corresponding changes in bacterial RNA, carbohydrate and protein contents were small.
DeanA. C. R.1962; Nucleic acid and protein content of Bacterium lactis aerogenes. 1. Equilibrium and non-equilibrium conditions. Proc. Roy. Soc. B 155:580
GaleE. F., EppsH. M. R.1942; The effect of the pH of the medium during growth on the enzymic activities of bacteria (Escherichia coli and Micrococcus lysodeikticus) and the biological significance of the changes produced. Biochem. J. 36:600
HerbertD.1961; The chemical composition of microorganisms as a function of their environment. Microbial Reaction to Environment. Symp. Soc. gen. Microbiol. 11:391
SchaechterM., MaaloeO., KjeldgaardN. O.1958; Dependency on medium and temperature of cell size and chemical composition during balanced growth of Salmonella typhimurium. J. gen. Microbiol. 19:592
SykesJ., TempestD. W.1965; The effect of magnesium and of carbon limitation on the macromolecular organisation and metabolic activity of Pseudomonas sp., Strain c-1 B. Biochim. Biophys. Acta 103:93
TempestD. W., HunterJ. R.1965; Influence of temperature on the macromolecular composition of C(glycerol)-limited and Mg2+-limited Aerobacter aerogenes, growing in a chemostat. J. gen. Microbiol. 39:vi
The Influence of Temperature and pH Value on the Macro-molecular Composition of Magnesium-limited and Glycerol-limited Aerobacter aerogenes Growing in a Chemostat