An Alternative Method of Isolating the Membrane of a Halophilic Bacterium Free

Abstract

SUMMARY

The membrane of was isolated after rupturing the organisms osmotically in 0.02 M-MgCl. Selected properties of this membrane were compared with those of membranes isolated from organisms which had been ruptured mechanically in 5 M-NaCl. The two types of preparation were indistinguishable from one another in the electron microscope, but some differences were noted between the present series of preparations and those described earlier. These differences, of which the occasional retention of the characteristic surface pattern of this organism was notable, were attributed to a slight modification of fixation conditions used in the present work. There were no major chemical differences between the two types of preparation but some minor differences in H titration and carbohydrate analyses possibly reflected slight structural differences between the two types of preparation. The osmotic method described here is much quicker than the mechanical one and, unlike the mechanical one, the time involved is largely independent of the quantity of organisms being used.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-41-2-225
1965-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/41/2/mic-41-2-225.html?itemId=/content/journal/micro/10.1099/00221287-41-2-225&mimeType=html&fmt=ahah

References

  1. Brown A. D. 1961; The peripheral structures of gram-negative bacteria. I. Cell wall protein and the action of a lytic enzyme system of a marine pseudomonad. Biochim. biophys. Acta 48:352
    [Google Scholar]
  2. Brown A. D. 1964; Aspects of bacterial response to the ionic environment. Bact. Rev. 28:296
    [Google Scholar]
  3. Brown A. D. 1965; Hydrogen ion titrations of intact and dissolved lipoprotein membranes. J. mol. Biol. 12:491
    [Google Scholar]
  4. Brown A. D., Shorey C. D. 1963; The cell envelopes of two extremely halophilic bacteria. J. Cell Biol. 18:681
    [Google Scholar]
  5. Dische Z. 1955; New color reagents for determination of sugars in polysaccharides. Meth. biochem. Analysis 2:313
    [Google Scholar]
  6. Fiske C. H., Subbarow Y. 1925; Colorimetric determination of phosphorus. J. biol. Chem. 66:375
    [Google Scholar]
  7. Houwink A. L. 1956; Flagella, gas vacuoles and cell-wall structure in Halobacterium halobium; an electron microscope study. J. gen. Microbiol. 15:146
    [Google Scholar]
  8. Kushner D. J., Bayley S. T., Boring J., Kates M., Gibbons N. E. 1964; Morphological and chemical properties of cell envelopes of the extreme halophile, Halo-bacterium cutirubrum. Can. J. Microbiol. 10:483
    [Google Scholar]
  9. Lowry O. H., Rosenbrotjgh N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem. 193:265
    [Google Scholar]
  10. Ponder E. 1961 The cell membrane and its properties. The Cell 21 Ed. by Brächet J., Mirsky A. E. New York: Academic Press Inc.;
    [Google Scholar]
  11. Rondle C. J. M., Morgan W. T. J. 1955; The determination of glucosamine and galactosamine. Biochem. J. 61:586
    [Google Scholar]
  12. Sehgal S. N., Gibbons N. E. 1960; Effect of some metal ions on the growth of Halo-bacterium cutirubrum. Can. J. Microbiol. 6:165
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-41-2-225
Loading
/content/journal/micro/10.1099/00221287-41-2-225
Loading

Data & Media loading...

Most cited Most Cited RSS feed