1887

Abstract

SUMMARY

Deficiency of meso-inositol (i-inositol, myo-inositol) caused decreased viability of ATCC 9080 and altered the internal morphology of the cells as seen in preparations stained with Nile Blue A, especially the nuclear membrane. Metabolic defects resulted which led to lipid accumulation in the organism and to increased concentrations of acetoin, acetaldehyde and glycerol in the culture medium. Hypotheses about the metabolic role of inositol are examined.

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/content/journal/micro/10.1099/00221287-41-2-215
1965-11-01
2022-06-29
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References

  1. Block R. J., Durrum E. L., Zweig G. 1958 Manual of Paper Chromatography and Paper Electrophoresis, 2nd ed. New York: Academic Press.;
    [Google Scholar]
  2. Challinor S. W., Daniels N. W. R. 1955; Fat production by inositol-deficient yeast. Nature, hond. 176:1267
    [Google Scholar]
  3. Challinor S. W., Power D. M., Tonge R. J. 1964; Effects of inositol-deficiency on yeast with particular reference to chemical composition of the cell and of the cell wall. Nature, Lond. 203:250
    [Google Scholar]
  4. Dawson R. M. C, White R. W., Freinkel N. 1962; The fate of meso-inositol during the growth of an inositol-dependent yeast Kloeckera brevis. J. gen. Microbiol. 57:331
    [Google Scholar]
  5. Deuel H. J. 1955 The Lipids 2675 New York: Interscience Publishers.;
    [Google Scholar]
  6. Diem K. 1962 Documenta Geigy, Scientific Tables470 New York: Geigy Corp., Ardsley.;
    [Google Scholar]
  7. Eagle M., Oyama V. I., Levy M., Freeman A. E. 1957; Myo-inositol as an essential growth factor for normal and malignant human cells in tissue culture. J. biol. Chem. 226:191
    [Google Scholar]
  8. Eastcott E. V. 1928; Wildier’s Bios. Isolation and identification of Bios I. J. Phys. Chem. 32:1094
    [Google Scholar]
  9. European Brewery Convention: Yeast Group 1962; Estimation of yeast viability. J. inst. Brew. 68:14
    [Google Scholar]
  10. Fink H., Kuhles R. 1933; Beiträge zur Methylenblaufärbung der Hefezellen und Studien über die Permeabilität der Hefezellmembran. Z. Physiol. Chem. 218:65
    [Google Scholar]
  11. Folch J., Lees M., Sloane-Stanley G. H. 1957; A simple method for the isolation and purification of total lipids from animal tissues. J. biol. Chem. 226:497
    [Google Scholar]
  12. Gancevici G., Stoian I., Keller Z. 1962; Localisation cytochimique des graisses dans les cultures de tissus. Archs. roum. Path. exp. Microbiol. 21:191
    [Google Scholar]
  13. Ghosh A., Charalampous F., Sison Y., Borer R. 1960; Metabolic functions of myo-inositol. I. Cytological and chemical alterations in yeast resulting from inositol deficiency. J. biol. Chem. 235:2522
    [Google Scholar]
  14. Hirsch J. 1963; Factice chromatography. J. Lipid Res. 4:1
    [Google Scholar]
  15. Keston A. S. 1956; 129th Meeting American Chemical Society. Abstract of Paper, 31C
  16. Lambert M., Neish A. C. 1950; Rapid method for estimation of glycerol in fermentation solutions. Can. J. Res. B 28:83
    [Google Scholar]
  17. Lewin L. M., Smith E. J. 1964; Accumulation of acetoin by myo-inositol deficient yeast. Nature, Lond. 203:867
    [Google Scholar]
  18. McKibbin J. M. 1959; The determination of inositol, ethanolamine, and serine in lipids. Meth. biochem. Analysis 7:111
    [Google Scholar]
  19. Neish A. C. 1952 Analytical Methods for Bacterial Fermentations37 Saskatoon: National Research Council of Canada.;
    [Google Scholar]
  20. Nord F. F., Weiss S. 1958 Cook A. H. ed. Chemistry and Biology of Yeasts339 New York: Academic Press.;
    [Google Scholar]
  21. Pedersen T. A. 1962; Lipid formation in Cryptococcus terricolus. III. Extraction and purification of lipids. Acta chem. scand. 16:374
    [Google Scholar]
  22. Ridgway G. J., Douglas H. C. 1958a; Distribution of inositol in subcellular fractions of yeast cells. J. Bact. 75:85
    [Google Scholar]
  23. Ridgway G. J., Douglas H. C. 1958b; Unbalanced growth of yeast due to inositol deficiency. J. Bact. 76:163
    [Google Scholar]
  24. Schopfer W. H., Posternak T., Wustenfeld D. 1962; Recherches sur le rôle du méso-inositol dans la biologie cellulaire de Schizosaccharomyces pombe Lindner. Arch. Microbiol. 44:113
    [Google Scholar]
  25. Shatkin A. J., Tatum E. L. 1961; The relationship of m-inositol to morphology in Neurospora crassa. Am. J. Bot. 48:760
    [Google Scholar]
  26. Smith R. H. 1951; A study of the role of inositol in the nutrition of Nematospora gos-sypii and Saccharomyces carlsbergensis. J. gen. Microbiol. 5:772
    [Google Scholar]
  27. Vignais P. M., Vignais P. V., Lehninger A. L. 1964; Identification of phospha-tidylinositol as a factor required in mitochondrial contraction. J. biol. Chem. 239:2011
    [Google Scholar]
  28. Westerfeld W. W. 1945; Colorimetric determination of blood acetoin. J. biol. Chem. 161:495
    [Google Scholar]
  29. Winge O., Roberts C. 1958 Chemistry and Biology of Yeasts ed. by Cook A. H.111 New York: Academic Press.;
    [Google Scholar]
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