1887

Abstract

SUMMARY

By using growth and division-inhibition at 16 hr as indices of activity, eight penicillins, differing in the side chain, were tested against an species, and compared with benzylpenicillin. DL-α-Aminobenzylpenicillin, DL-3-chloro-α-aminobenzylpenicillin, D-α-aminobenzylpenicillin, and methicillin were more toxic for growth than was benzylpenicillin. 6-Aminopenicillanic acid and cloxacillin were about equal to benzylpenicillin in toxicity; triphenylmethylpenicillin and 2-ethoxy-1-naphthylpenicillin were much less toxic. All the compounds inhibited division of the bacteria but, at 50% of normal growth, methicillin, cloxacillin and 2-ethoxy-1-naphthylpenicillin resulted in significantly longer organisms than the others. Pantoyl lactone, when present in the medium from the time of inoculation, in all cases decreased the length of the organisms, increased growth (with a highly toxic concentration of penicillin), and decreased the accumulation of keto acids and ultraviolet-absorbing materials in the medium. It was concluded that the side chain of these penicillins is not essential for the inhibition of growth and division of this sp.; quantitative differences in activity associated with the side chain appear to result from the influence of the side chain on factors such as penetration, or strength of binding at the active site.

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/content/journal/micro/10.1099/00221287-41-2-155
1965-11-01
2022-05-21
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References

  1. Abraham E. P., Crawford K., Newton G. F., Burton H. S., Hale E. W. 1953; Cephalosporin N: a new type of penicillin. Nature, Lond. 171:343
    [Google Scholar]
  2. Boman H. G., Eriksson K. G. 1963; Penicillin-induced lysis in Escherichia coli. J. gen. Microbiol. 31:339
    [Google Scholar]
  3. Collins J. F., Richmond M. H. 1962; A structural similarity between N-acetyl-muramic acid and penicillin as a basis for antibiotic action. Nature, Lond. 195:142
    [Google Scholar]
  4. Cooper P. D. 1956; Site of action of radioactive penicillin. Bact. Rev. 20:28
    [Google Scholar]
  5. Grula E. A. 1960a; Cell division in a species of Erwinia. I. Initial observations relating to nutritional dependency. J. Bact. 80:369
    [Google Scholar]
  6. Grula E. A. 1960b; Cell division in a species of Erwinia. II. Inhibition of division by d-amino acids. J. Bact. 80:375
    [Google Scholar]
  7. Grula E. A., Grula M. M. 1962; Cell division in a species of Erwinia. III. Reversal of inhibition of cell division caused by d-amino acids, penicillin, and ultraviolet light. J. Bact. 83:981
    [Google Scholar]
  8. Grula E. A., Grula M. M. 1964; Cell division in a species of Erwinia. VII. Amino sugar content of dividing and non-dividing cells. Biochem. Biophys. Res. Coram. 17:341
    [Google Scholar]
  9. Haidle C. W., Knight S. G. 1960; A colorimetric assay for 2-heptanone and other ketones. Biochim. biophys. Acta 39:536
    [Google Scholar]
  10. Hugo W. B., Russell A. D. 1960; Action of 6-aminopenicillanic acid on Gram negative bacteria. Nature, Lond. 188:875
    [Google Scholar]
  11. Mohberg J., Johnson M. J. 1958; Comparative study of the binding of synnematin and penicillin by bacterial cells. J. Bact. 76:385
    [Google Scholar]
  12. Pollock M. R. 1962 Penicillinase. Resistance of Bacteria to the Penicillins.CIBA Foundation Study Group1356 London: J. and A. Churchill Ltd.;
    [Google Scholar]
  13. Rogers H. J., Mandelstam J. 1962; Inhibition of cell-wall-mucopeptide formation in Escherichia coli by benzylpenicillin and 6-(d( — )-α-Aminophenylacetamido) penicillanic acid (‘ampicillin’). Biochem. J. 84:299
    [Google Scholar]
  14. Rolinson G. N. 1962 cf. Discussion on mode of action of the penicillins, by H. J. Rogers. Resistance of Bacteria to the PenicillinsCIBA Foundation Study Group1347 London: J. and A. Churchill Ltd.;
    [Google Scholar]
  15. Rolinson G. N., Stevens S. 1961; Microbiological studies on a new broad-spectrum penicillin, ‘Penbritin’. Br. med. J. ii:191
    [Google Scholar]
  16. Russell A. D. 1962; A note on the activity of six penicillins against Escherichia coli. J. Pharm., Lond. 14:390
    [Google Scholar]
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