1887

Abstract

SUMMARY:

The effect of X-rays on the synthesis of induced β-D-galactosidase was examined with strain B and three of its mutants, i.e. the sensitive strain BS and two resistant strains B/r and B/H. With the parent strain B the extent of radiation-induced lethal damage depended on conditions of culture after irradiation, whereas radiation-induced inhibition of β-D-galactosidase was unaffected by the composition of the culture medium. The sensitivity of radiation damage to induction of β-D-galactosidase was about the same for strains B, B/r and B/H, the dose required to give 37% survival being 42 ± 6 krad; in BS the sensitivity to radiation was three times as great. Enzyme induction was one-half to one-third as radiosensitive as viability for the strains B/r, B/H and BS but with strain B it was one-tenth as sensitive when Oxoid Nutrient Medium was used. Oxygen enhancement ratios for lethal effects were always smaller than those for inhibition of enzyme synthesis.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-41-1-119
1965-10-01
2024-10-05
Loading full text...

Full text loading...

/deliver/fulltext/micro/41/1/mic-41-1-119.html?itemId=/content/journal/micro/10.1099/00221287-41-1-119&mimeType=html&fmt=ahah

References

  1. Alper T. 1955; Bacteriophage as indicator in radiation chemistry. Radiat. Res. 2:119
    [Google Scholar]
  2. Alper T. 1961; Variability of the oxygen effect observed with micro-organisms. Part II. Escherichia coli B. Int. J. Radiat Biol. 3:369
    [Google Scholar]
  3. Alper T. 1963; Lethal mutations and cell death. Physics Med. Biol. 8:365
    [Google Scholar]
  4. Alper T., Gillies N. E. 1958a; Restoration of Escherichia coli strain b after irradiation: its dependence on suboptimal growth conditions. J. gen. Microbiol. 18:461
    [Google Scholar]
  5. Alper T., Gillies N. E. 1958b; ‘Restoration’ of micro-organisms and suboptimal growth conditions. Radiat. Res. 9:86
    [Google Scholar]
  6. Alper T., Gillies N. E. 1960a The influence of metabolic events immediately after irradiation on the difference in sensitivity between E. coli b and E. coli b/r. ‘Immediate and low level effects of ionizing radiations’Proceedings of Symposium held at VeniceJune 22-26,1959 Edited by Buzzati-Traverso A. A.205 London: Taylor & Francis, Ltd.;
    [Google Scholar]
  7. Alper T., Gillies N. E. 1960b; The relationship between growth and survival after irradiation of Escherichia coli strain b and two resistant mutants. J. gen. Microbiol. 22:113
    [Google Scholar]
  8. Anderson E. H. 1951; The effect of oxygen on mutation induced by X-rays. Proc. natn. Acad. Sci., U.S.A. 37:340
    [Google Scholar]
  9. Billen B., Lichstein H. C. 1952; The effect of X-radiation on the adaptive formation of formic hydrogenlyase in Escherichia coli. J. Bact. 63:533
    [Google Scholar]
  10. Bridges B. A. 1963; Effect of chemical modifiers on inactivation and mutation by gamma radiation in Escherichia coli. J. gen. Microbiol. 31:405
    [Google Scholar]
  11. Deering R. 1963; Mutations and killing of Escherichia coli wp-2 by accelerated heavy ions and other radiations. Radiat. Res. 19:169
    [Google Scholar]
  12. Dewey D. L. 1962; X-ray inactivation of inducible enzyme synthesis and the effect of oxygen and glycerol. Nature, hond. 194:158
    [Google Scholar]
  13. Gillies N. E., Alper T. 1959; Reductions in the lethal effects of radiations on Escherichia coli b by treatment with chloramphenicol. Nature, Lond. 183:237
    [Google Scholar]
  14. Gros F. 1960 The Nucleic Acids vol. in 409 Ed. Chargaff E., Davidson J. N. New York: Academic Press.;
  15. Hodgkins B., Alper T. 1963; Anaerobic growth as a factor influencing radio-sensitivity. J. gen. Microbiol. 30:307
    [Google Scholar]
  16. Howard-Flanders P., Levin J., Theriot L. 1963; Reaction of desoxyribonucleic acid radicals with sulphydryl compounds in X-irradiated bacteriophage systems. Radiat. Res. 18:593
    [Google Scholar]
  17. Jacob F., Monod J. 1961; Genetic regulatory mechanisms. J. molec. Biol. 3:318
    [Google Scholar]
  18. Kameyama T., Novelli G. D. 1962; Effect of ultraviolet inactivation and photo-activation on the induced synthesis of β-galactosidase by Escherichia coli. Arch. Biochem. Biophys. 97:529
    [Google Scholar]
  19. Kioh Y., Rich A. 1964; Induced enzyme formed on bacterial polyribosomes. Proc. natn. Acad. Sci., V S.A. 51:111
    [Google Scholar]
  20. Lea D. E. 1946 Actions of Radiation on Living Cells. Cambridge University Press;
  21. Lederberg J. 1950; β-d-galactosidase of E. coli κ 12. J. Bact. 60:381
    [Google Scholar]
  22. Novelli G. D., Kameyama T., Eisenstadt J. M. 1961; The effect of ultraviolet light and X-rays on an enzyme forming system. J. cell. comp. Physiol. 58:225
    [Google Scholar]
  23. Pauly H. 1959; X-ray sensitivity and target volume of enzyme induction. Nature, Lond. 184:1570
    [Google Scholar]
  24. Pauly H. 1963; Synthesis of protein, RNA, DNA and induced enzyme in Bacterium cadaveris after X-irradiation. Int. J. Radiat. Biol. 6:221
    [Google Scholar]
  25. Pollard E. C., Barrett N. 1959; The radiation sensitivity and molecular weight of dry β-galactosidase. Radiat. Res. 11:781
    [Google Scholar]
  26. Pollard E. C, Guild W. R., Hutchinson F., Setlow R. B. 1955; The direct action of ionising radiation on enzymes and antigens. Prog. Biophys. biophys. Chem. 5:72
    [Google Scholar]
  27. Pollard E. C., Vogler C. 1961; Radiation action on some metabolic processes in Escherichia coli. Radiat. Res. 15:109
    [Google Scholar]
  28. Swenson P. A., Setlow R. B. 1964; β-Galactosidase : inactivation of its messenger RNA by ultraviolet irradiation. Science 146:791
    [Google Scholar]
/content/journal/micro/10.1099/00221287-41-1-119
Loading
/content/journal/micro/10.1099/00221287-41-1-119
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error