%0 Journal Article %A RAJADHYAKSHA, A. B. %A SRINIVASA RAO, S. %T The Role of Phage in the Transduction of the Toxinogenic Factor in Corynebacterium diphtheriae %D 1965 %J Microbiology, %V 40 %N 3 %P 421-429 %@ 1465-2080 %R https://doi.org/10.1099/00221287-40-3-421 %I Microbiology Society, %X SURMMARY Strains of Corynebacterium diphtheriae which produced large amounts of toxin (e.g. pw8) were found to be non-lysogenie, but they contained a cytoplasmic toxinogenic factor designated as T+. After infection with phage they produced less toxin. It is possible to remove the phage from the lysogenic strains without affecting the T+ factor by treatment with small amounts of acriflavine. When treated with larger amounts of acriflavine the T+ factor was also removed, rendering the strain non-toxinogenic. The toxin-inducing phages can be converted in vitro into non-toxinogenic phages by treatment with small amounts of acriflavine. After passage through the pw8t + strains these phages revert to toxin-inducing ability. Similarly, the naturally occurring, non-toxinogenic γ phage can be made to induce toxin production. This indicates that the role of phage in diphtheria toxin production is to transduce the T+ factor. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-40-3-421