Strains of which produced large amounts of toxin (e.g. pw8) were found to be non-lysogenie, but they contained a cytoplasmic toxinogenic factor designated as T. After infection with phage they produced less toxin. It is possible to remove the phage from the lysogenic strains without affecting the T factor by treatment with small amounts of acriflavine. When treated with larger amounts of acriflavine the T factor was also removed, rendering the strain non-toxinogenic. The toxin-inducing phages can be converted into non-toxinogenic phages by treatment with small amounts of acriflavine. After passage through the pw8T strains these phages revert to toxin-inducing ability. Similarly, the naturally occurring, non-toxinogenic γ phage can be made to induce toxin production. This indicates that the role of phage in diphtheria toxin production is to transduce the T factor.


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