1887

Abstract

SURMMARY

Strains of which produced large amounts of toxin (e.g. 8) were found to be non-lysogenie, but they contained a cytoplasmic toxinogenic factor designated as T. After infection with phage they produced less toxin. It is possible to remove the phage from the lysogenic strains without affecting the T factor by treatment with small amounts of acriflavine. When treated with larger amounts of acriflavine the T factor was also removed, rendering the strain non-toxinogenic. The toxin-inducing phages can be converted into non-toxinogenic phages by treatment with small amounts of acriflavine. After passage through the 8 strains these phages revert to toxin-inducing ability. Similarly, the naturally occurring, non-toxinogenic phage can be made to induce toxin production. This indicates that the role of phage in diphtheria toxin production is to transduce the T factor.

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1965-09-01
2022-01-23
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References

  1. Adams M. H. 1950; Section I. Methods of study of bacterial viruses. Meth. med. Res 2:7
    [Google Scholar]
  2. Adams M. H. 1959; Lysogeny. Bacteriophages375 New York: Interscience Publishers Inc;
    [Google Scholar]
  3. Asheshov E. A., Jevons M. P. 1963; The effect of heat on the ability of a host strain to support the growth of a Staphylococcus phage. J. gen. Microbiol 31:97
    [Google Scholar]
  4. Barksdale L., Garmise L., Horibata K. 1960; Virulence, toxinogeny and lysogeny in Corynebacterium diphtheriae. Ann. N. Y. Acad. Sci 88:1093
    [Google Scholar]
  5. Barksdale L., Garmise L., Rivera R. 1961; Toxinogeny in Corynebacterium diphtheriae. J. Bact 81:527
    [Google Scholar]
  6. Barksdale L., Pappenheimer A. M. Jun. 1954; Phage-host relationships in non-toxinogenic and toxinogenic diphtheria bacilli. J. Bact 67:220
    [Google Scholar]
  7. Brenner S., Barnett L., Crick F. H. C., Orgel A. 1961; The theory of mutagenesis. J. mol. Biol 3:121
    [Google Scholar]
  8. Freeman V. J. 1951; Studies on the virulence of bacteriophage-infected strains of Corynebacterium diphtheriae. J. Bact 61:675
    [Google Scholar]
  9. Freeman V. J., Morse I. U. 1952; Further observations on the change to virulence of bacteriophage-infected avirulent strains of Corynebacterium diphtheriae. J. Bact 63:407
    [Google Scholar]
  10. Groman N. B. 1953a; The relation of bacteriophage to the change of Corynebacterium diphtheriae from avirulence to virulence. Science 117:297
    [Google Scholar]
  11. Groman N. B. 1953b; Evidence for the induced nature of the change from nontoxigenicity to toxigenicity in Corynebacterium diphtheriae as a result of exposure to specific bacteriophage. J. Bact 66:184
    [Google Scholar]
  12. Groman N. B. 1955; Evidence for the active role of bacteriophage in the conversion of nontoxigenic Corynebacterium diphtheriae to toxin production. J. Bact 69:9
    [Google Scholar]
  13. Groman N. B., Eaton M. 1955; Genetic factors in Corynebacterium diphtheriae conversion. J. Bact 70:637
    [Google Scholar]
  14. Hirota Y. 1960; The effect of acridine dyes on mating type factors in Escherichia coli. Proc. natn. Acad. SciU. S. A 4657
    [Google Scholar]
  15. Hirota Y., Iijima T. 1957; Acriflavine as an effective agent for eliminating F factor in Escherichia coli k-12. Nature, Lond 180:655
    [Google Scholar]
  16. Lederberg E. M., Lederberg J. 1953; Genetic studies of lysogenicity in inter-strain crosses in Escherichia coli. Genetics 38:51 Zabriskie J. B. 1964; The role of temperate bacteriophage in the production of erythrogenic toxin by group A streptococci. J. exp. Med 119:761
    [Google Scholar]
  17. Linggood F. V., Fenton E. L. 1947; The production of diphtheria toxin by submerged culture in shaking flasks. Appendix: Preparation of culture media for the production of diphtheria toxin. Br. J. exp. Path 28:854
    [Google Scholar]
  18. Pappenheimer A. M. Jun., Howland J., Miller P. A. 1962; Electron transport systems in Corynebacterium diphtheriae. Biochim. biophys. Acta 64:229
    [Google Scholar]
  19. Pappenheimer A. M. Jun., Miller M., Yoneda M. 1962; Kinetics of diphtheria toxin formation. J. gen. Microbiol 28:531
    [Google Scholar]
  20. Sneath P. H. A. 1962; Sex factors as episomes. Br. med. Bull 18:41
    [Google Scholar]
  21. Wohlhieter J. A., Palkow S., Citrella R. V., Baron L. S. 1964; Characterization of DNA from a Proteus strain harbouring an episome. J. mol. Biol 9:576
    [Google Scholar]
  22. World Health Organization 1953 Technical Report Series61
    [Google Scholar]
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