1887

Abstract

SURMMARY

Samples of deoxyribonucleic acid (DNA), microsomal ribonucleic acid (RNA) and a neutral polysaccharide from var. were isolated and analysed. The DNA was found to have an adenine + thymine/guanine + cytosine value of 3·04, and the RNA to have an adenine+ uracil/guanine + cytosine value of 1·21.

The amino acid composition of the protein of the organism was also determined and found to be in close agreement with the values expected by extrapolating the curves obtained from the amino acid contents of the protein of several bacteria having DNA’s with a wide range of adenine + thymine/guanine + cytosine values ( Sueoka, 1961 ). The values were also in close agreement with those obtained for the protein of , which has a DNA of similar base composition (A + T/G + C = 3·0). Two significant exceptions were noted: the value for proline was almost twice as large as would have been expected and a large amount of cysteine was found.

The mycoplasma organisms contained a very small amount of a neutral polysaccharide in which only glucose was detected.

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1965-09-01
2024-03-28
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References

  1. Dische Z. 1955; Color reactions of nucleic acid components. In The Nucleic Acids 1285 Charaff E., Davidson J. N. New York: Academic Press;
    [Google Scholar]
  2. Euler H., Hahn L. A. 1946; A new method for the quantitative determination of ribonucleic acid in animal tissues. Svensk. kern. Tidskr 10:251
    [Google Scholar]
  3. Fraser D. 1957; Comparison of the amino acid composition of T2 and T3 bacterio- phages. J. biol. Chem 227:711
    [Google Scholar]
  4. Jones A. S., Lee W. A., Peacocke A. R. 1951; The determination of phosphorus in deoxypentose nucleic acids. J. chem. Soc623
    [Google Scholar]
  5. Jones A. S., Walker R. T. 1963a; Isolation and analysis of the deoxyribonucleic acid of Mycoplasma mycoides var. capri. Nature, Lond 198:588
    [Google Scholar]
  6. Jones A. S., Walker R. T. 1963b; Studies on the deoxyribonucleic acid of Serratia marcescens. J. gen. Microbiol 31:187
    [Google Scholar]
  7. Kirby K. S. 1955; Some new solvent systems for the paper chromatography of nucleic acid degradation products. Biochim. biophys. Acta 18:575
    [Google Scholar]
  8. Lynn R. J., Smith P. F. 1957; Nucleic acid content of pleuropneumonia-like organisms from human sources. J. Bact 74:811
    [Google Scholar]
  9. Lynn R. J., Smith P. F. 1960; Chemical composition of PPLO. Ann. N.Y. Acad. Sci 79:493
    [Google Scholar]
  10. Markham R., Smith J. D. 1951; Chromatographic studies of nucleic acids. 4. The nucleic acids of the tobacco yellow mosaic virus including a note on the nucleic acids of the tomato bushy stunt virus. Biochem. J 49:401
    [Google Scholar]
  11. Morowitz H. J., Tourtellotte M. E., Guild W. R., Castro E., Woese C., Cleverdon R. C. 1962; The chemical composition and submicroscopic morphology of Mycoplasma gallisepticum, avian pplo 5969. J. molec. Biol 4:93
    [Google Scholar]
  12. Partridge S. M. 1949; Aniline hydrogen phthalate as a spraying reagent for chromatography of sugars. Nature, Lond 164:443
    [Google Scholar]
  13. Plackett P., Buttery S. H. 1958; A galactan from Mycoplasma mycoides. Nature, Lond 182:1236
    [Google Scholar]
  14. Salton M. R. J. 1960; Surface layers of the bacterial cell. In The Bacteria 1105 Gunsalus I. C., Stanier R. R. New York: Academic Press;
    [Google Scholar]
  15. Scherbaum O. 1957; The content and composition of nucleic acids in normal and synchronously dividing mass cultures of Tetrahymena pyriformis. Exptl Cell Res 13:24
    [Google Scholar]
  16. Spackmann D. H., Stein W. H., Moore S. 1958; Automatic recording apparatus for use in the chromatography of amino acids. Anal. Chem 30:1190
    [Google Scholar]
  17. Sueoka N. 1961; Correlation between base composition of deoxyribonucleic acid and amino acid composition of protein. Proc. natn. Acad. SciU.S.A 471141
    [Google Scholar]
  18. Trevelyan W. E., Procter D. P., Harrison J. S. 1950; Detection of sugars on paper chromatograms. Nature, Lond 166:444
    [Google Scholar]
  19. Wyatt G. R. 1951; The purine and pyrimidine composition of deoxypentosenucleic acids. Biochem. J 48:584
    [Google Scholar]
  20. Wyatt G. R., Cohen S. S. 1953; The bases of the nucleic acids of some bacterial and animal viruses: the occurrence of 5-hydroxymethylcytosine. Biochem. J 55:774
    [Google Scholar]
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