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1887

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Volume 40, Issue 3

Carbohydrate Metabolism of Iron-Rich and Iron-Poor Free

Abstract

SUMMARY

Comparative studies were made of the metabolic activities of a strain of grown in iron-poor and iron-rich trypticase medium, with and without glucose. Four nutritionally distinctive types of organisms were produced: iron-rich without glucose (Fe + G –); iron-poor without glucose (Fe– G –); iron-rich with glucose (Fe + G + ); iron-poor with glucose (Fe– G+). Fe+ G– cocci oxidized glucose, and -lactate, pyruvate, acetate, formate, and Krebs cycle intermediates to completion. Of these substrates, only glucose and the lac-tates were oxidized by the Fe– G– organisms. These latter oxidations proceeded at decreased rates and incompletely to acetate and acetoin. Fe+ G+ cocci in comparison with the Fe+ G– cocci showed glucose inhibition of oxidative capacity by metabolizing only glucose, L-lactate and pyruvate to acetate and failing to oxidize -lactate, acetate and Krebs cycle intermediates. The Fe– G+ cocci showed the most severe restriction of oxidative capacity by oxidizing only glucose with the accumulation of much lactate and minor amounts of pyruvate, acetate and acetoin. The Fe – G –, Fe – G +, and Fe + G + cocci glycolysed glucose at comparable rates, while the highly oxidative form Fe+ G– showed a markedly decreased glycolytic activity. The catalase activity of both types of iron-poor cocci was very much lower than that of the iron-rich organisms. Of the latter, the Fe+ G+ cocci showed a catalase activity only 20% that of Fe + G – cocci.

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© Society for General Microbiology 1965
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1965-09-01
2025-01-17
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References

  1. Barker H. A., Summerson J. H. 1941; The colorimetric determination of lactic acid in biological material. J. biol. Chem 138:535
     [Google Scholar]
  2. Collins F. M., Lascelles J. 1962; The effect of growth conditions on oxidative and dehydrogenase activity in Staphylococcus aureus . J. gen. Microbiol 29:531
     [Google Scholar]
  3. Frtedemann F. M., Haugen G. E. 1943; Pyruvic acid. II. The determination of keto acids in blood and urine. J. biol. Chem 147:415
     [Google Scholar]
  4. Gershanovitch V. N., Palkina N. A., Burd G. I. 1963; The role of lactate in regulation of the enzymatic system synthesis participating in the acetate oxidation of Staphylococcus aureus . Biochem. biophys. Res. Commun 13:12
     [Google Scholar]
  5. O’Kane B. G. 1954; Non-lipoic systems: acetate formation by Proteus and acetyl phosphate formation by Clostridium . Fed. Proc 13739
     [Google Scholar]
  6. Schade A. L. 1960 Protides of Biological Fluids261Proc. 8th Colloq. Bruges Amsterdam: Elsevier Publishing Co;
     [Google Scholar]
  7. Schade A. L. 1963; Significance of serum iron for the growth, biological characteristics, and metabolism of Staphylococcus aureus . Biochem. Z 338:140
     [Google Scholar]
  8. Schade A. L., Caroline L. 1944; Raw hen egg white and the role of iron in growth inhibition of Shigella dysenteriae, Staphylococcus aureus, Escherichia coli, and Sac- charomyces cerevisiae . Science 100:14
     [Google Scholar]
  9. Schade A. L., Oyama J., Reinhart R. W., Miller J. R. 1954; Bound iron and unsaturated iron-binding capacity of serum ; rapid and reliable quantitative determination. Proc. Soc. Exp. Biol. Med 87443
     [Google Scholar]
  10. Soodak M. 1957; An enzymatic micromethod for the determination of acetate. Meth. Enzymol 3:266
     [Google Scholar]
  11. Stedman R. L., Kravitz E. 1955; Evidence for a common pathway for pyruvate and acetate oxidation by Micrococcus pyogenes var. aureus. Archs. Biochem. Biophys 59:260
     [Google Scholar]
  12. Strasters K. C., Winkler K. C. 1963; Carbohydrate metabolism in Staphylococcus aureus . J. gen. Microbiol 33:213
     [Google Scholar]
  13. Sutton W. B. 1954; Isolation and properties of a lactic oxidative decarboxylase from Mycobacterium phlei . J. biol. Chem 210:309
     [Google Scholar]
  14. Theodore T. S., Schade A. L. 1965; Growth of Staphylococcus aureus in media of restricted and unrestricted inorganic iron availability. J. gen. Microbiol 39:75
     [Google Scholar]
  15. Umbreit W. W., Burris R. H., Stauffer J. F. 1957 Manometric Techniques, 3. Minneapolis: Burgess Publishing Co;
     [Google Scholar]
  16. Waring W. S., Werkman C. H. 1944; Iron deficiency in bacterial metabolism. Arch. Biochem 4:75
     [Google Scholar]
  17. Webley D. M., Duff R. B., Anderson G. 1962; The metabolism of iron-, zinc-, and manganese-deficient Nocardia opaca . J. gen. Microbiol 29:179
     [Google Scholar]
  18. Westerfeld W. W. 1945; A colorimetric determination of blood acetoin. J. biol. Chem 161:495
     [Google Scholar]
/content/journal/micro/10.1099/00221287-40-3-385
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Carbohydrate Metabolism of Iron-Rich and Iron-Poor Staphylococcus aureus
Microbiology 40, 385 (1965); https://doi.org/10.1099/00221287-40-3-385
/content/journal/micro/10.1099/00221287-40-3-385
/content/journal/micro/10.1099/00221287-40-3-385
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