1887

Abstract

SUMMARY

The morphology of was well preserved after washing and suspension in buffered 0·4 -sucrose solutions, but the survival of viable particles was no better, and the loss of ultraviolet (u.v.)-absorbing substances and the decrease of turbidity was no less than in hypotonic solution (0·01 -tris HCl or 0·01 -NaHPO + KHPO). The addition of Mg, Ca, spermidine or spermine (increasing order of activity) decreased the decrease of turbidity and loss of u.v-absorbing substances. Ca+ and Mg, but not spermine, increased the degree of survival of viable particles. Ethylenediaminetetra-acetate (EDTA; 0·01 ) increased the loss of u.v.-absorbing substances, and decreased the turbidity and degree of survival. Ca, Mg and spermine annulled the effects of EDTA on loss of u.v.-absorbing material and on the turbidity, but only Ca prevented the lethal effect of EDTA. Filaments disappeared and cell volume increased when the organisms were transferred from hypertonic to hypotonic solutions ; the shape changes were reversible.

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/content/journal/micro/10.1099/00221287-40-2-227
1965-08-01
2021-07-27
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