Lysostaphin attacked both viable staphylococci and the mucopeptide portion of the staphylococcal cell wall. In the absence of salts, lysostaphin activity could only be recovered from the particulate portion of the lysed cell after centrifugation, whereas in the absence of salts its action on the mucopeptide resulted in a recovery of active material in both the sediment and the supernatant fluid. It appears from these observations that lysostaphin is complexed with its substrate and that NaCl is required to break the complex.
LitwackG.1960Lysozyme as Related to Problems in MicrobiologySymposium of the 1960 Annual Meeting of the Society of American BacteriologistsWest Lafayette, Indiana, Purdue University
MoraP. T.,
YoungB. G.,
ShearM. J.1959; Reduction of toxicity of cationic macromolecules by complexing with anionic derivatives of synthetic polyglucoses. Nature, Lond. 184:431
ParkJ. T.1961; Mucopeptides of Bacterial Cell Walls. In Immunochemical Approaches to Problems in MicrobiologyHeidelbergerM.,
PlesciaO. J.
New Brunswick, N. J.: Rutgers University Press;
PerkinsH. R.,
RogersH. J.1959; The products of partial acid hydrolysis of the mucopeptide from cell walls of Micrococcus lysodeikticus
. Biochem. J 72:647
SchindlerC. A.,
SchuhardtV. T.1965; Purification and properties of lysostaphin. A lytic agent for Staphylococcus aureus
. Biochim. biophys. Acta 97:242
Weibuix C,
ZachariasB.,
BeckmanH.1959; Affinity of lysozyme to structural elements of the bacterial cell as studied with enzyme labelled with radioactive iodine. Nature, Lond. 184:1744