SUMMARY: The life-cycle of actinophage MSP 2 in host s was divisible into distinct stages by manipulating the cultural conditions and by the use of certain chemical compounds. Two types of attachment were observed: reversible and irreversible. Reversible attachment occurred instantaneously and was relatively independent of temperature and phage concentration. The controlling variables in extent of adsorption were the amount of host mycelium and ionic content of the medium. Irreversible attachment was time dependent and temperature dependent. Addition of acriflavine allowed phage attachment and injection to occur, but suppressed some stage of intracellular growth or maturation. Acriflavine prevented formation of viable phage, but phage components were demonstrated immunologically and electron-microscopically. Chilling of phage-infected cultures allowed lysis to proceed, with release of preformed phages; but no new phages were formed. These results indicate that the life-cycle of actinophage MSP 2 is experimentally separable into attachment, injection, growth, maturation and lysis.


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