1887

Abstract

SUMMARY

After incubation for 1 hr at 37° with caseinase C (a purified fraction of actinomycetin), the neuraminidase activity of concentrated purified influenza virus 8 suspension was unchanged, whereas its infectivity and haemagglutinating activity were considerably decreased. After 4 hr, infectivity and haemagglutinating activity were still more decreased and most of the neuraminidase activity was destroyed. The ability to fix complement in the presence of specific antibodies was slightly decreased, whereas the ability to neutralize haemagglutination-inhibiting antibodies was not affected.

Influenza virus 8 suspension treated with enzyme caseinase C contained material sedimentable by centrifugation for 1 hr at 31,000 and material which remained in the supernatant fluid under these conditions. Both materials fixed complement in the presence of control 8 virus antiserum. The ability of control 8 virus suspension to neutralize haemagglutination-inhibiting antibodies and its ability to react with strain-specific complement-fixing antibodies were related to material sedimentable by centrifugation for 1 hr at 31,000 . Treatment of PR8 virus suspension by caseinase C destroyed its ability to produce antibody-fixing complement in the presence of control influenza virus 8. But this treatment did not suppress ability to produce specific haemagglutination-inhibiting antibodies. Following this treatment of 8 virus a new antigenic activity was shown: the antiserum to enzyme-treated virus not only fixed complement in the presence of enzyme-treated 8 virus, but also in the presence of enzyme-treated Asian virus. Material which fixed complement in the presence of antiserum to enzyme-treated virus was sedimentable from enzyme-treated 8 virus suspension by centrifugation at 31,000 .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-40-2-157
1965-08-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/40/2/mic-40-2-157.html?itemId=/content/journal/micro/10.1099/00221287-40-2-157&mimeType=html&fmt=ahah

References

  1. Boyd W. C. 1956 Fundamentals of Immunology, 3.704 New York: Inter-science Publishers Inc;
    [Google Scholar]
  2. Cleeland R., Sugg J. Y. 1963; Inactivation of hemagglutinating capacity of type A influenza virus by trypsin treatment. Proc. Soc. exp. Biol. Med 112913
    [Google Scholar]
  3. Dierickx L., Ghuysen J. M. 1962; Purification de la β-(1-4)-N-acetylhexosaminidase sécrétée par Streptomyces albus g et active sur les parois de Micrococcus lysodeikticus . Biochim. biophys. Acta 58:7
    [Google Scholar]
  4. Fazekas de St Groth S., White D. O. 1958; An improved assay for the infectivity of influenza viruses. J. Hyg., Camb 56:151
    [Google Scholar]
  5. Finney D. J. 1947 Probit Analysis Cambridge University Press;
    [Google Scholar]
  6. Fulton F., Dumbell K. R. 1949; The serological comparison of strains of influenza virus. J. gen. Microbiol 3:97
    [Google Scholar]
  7. Ghuyser J. M., Leyh-Bouille M., Dierickx L. 1962; Structure des parois de Bacillus megaterium kmi Isolement de l’amidase et d’un enzyme nouveau sécrété par Streptomyces albus g et actif sur les parois de Bacillus megaterium km et de Micrococcus lysodeikticus . Biochim. biophys. Acta 63:286
    [Google Scholar]
  8. Gresser I., Enders J. F. 1961; The effect of trypsin on representative myxoviruses. Virology 13:420
    [Google Scholar]
  9. Henle W., Lief F. S., Fabiyi A. 1958; Strain specific complement fixation test in antigenic analysis and serodiagnosis of influenza. Lancet i:818
    [Google Scholar]
  10. Henle W., Wiener M. 1944; Complement fixation antigens of influenza viruses type A and B. Proc. Soc. exp. Biol. Med 57:176
    [Google Scholar]
  11. Hobson D., Pearson E. 1961; Serological responses to Asian influenza viruses in man : a comparison of haemagglutination-inhibition and complement fixation methods. Br. J. exp. Path 42:53
    [Google Scholar]
  12. Kuroya M., Hinuma Y., Higo N., Ishihara K., Kikuchi Ko., Kaneko T., Kobayashi N., Anzai A. 1957; Studies on antiviral antibiotics produced by Streptomyces. IV. Screening of antibiotics against influenza virus in vitro . Jap. J. Microbiol 1:49
    [Google Scholar]
  13. Lief F. S. 1963; Antigenic analysis of influenza viruses by complement fixation. VII. Further studies on production of pure anti-S serum and on specificity of type AS antigens. J. Immun 90:172
    [Google Scholar]
  14. Malchair R. 1958; Effets de l’actinomycétine sur le virus grippal. G. Microbiol 5:137
    [Google Scholar]
  15. Mayrow L. W., Robert B., Winzler R. J., Rafelson M. E. 1961; Studies on the neuraminidase of influenza virus. I. Separation and some properties of the enzyme from Asian and pr 8 strains. Archs. Biochem. Biophys 92:475
    [Google Scholar]
  16. Skryabin G. K. 1957; Virusin 1609. A new antibiotic of actinomycetes derivation. (Russian text.). Antibiotiki 1:10
    [Google Scholar]
  17. Sohier R., Peillard M. M., Gineste J., Freydier J. 1956; Mierométhode en tubes pour la réaction de fixation du complément appliquée au diagnostic des infections à virus. Annls. Biol. clin 14:1
    [Google Scholar]
  18. Stone J. D. 1949; Tryptic inactivation of the RDE of Vibrio cholerae and of the enzymic activity of influenza virus. Aust. J. exp. Biol. med. Sci 27:229
    [Google Scholar]
  19. Tamm I., Horsfall F. L. Jr 1950; Characterization and separation of an inhibitor of viral haemagglutination present in urine. Proc. Soc. exp. Biol. Med 74108
    [Google Scholar]
  20. Warren L. 1959; The thiobarbituric acid assay of sialic acids. J. biol. Chem 234:1971
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-40-2-157
Loading
/content/journal/micro/10.1099/00221287-40-2-157
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error