1887

Abstract

SUMMARY

Cellular components in homogenates of brain tissue from scrapieaffected mice have been separated by centrifugation in sucrose and caesium chloride density gradients with the objective of location of the scrapie agent and concentration of scrapie activity. Improvements in the relative activity of the fractions removed from sucrose gradients were small but recovery was high in the material sedimented through 0·88 -sucrose. No peak of activity was observed in zones removed from a caesium chloride gradient and activity throughout remained strongly associated with particulate matter. Ultrasonic disruption had little effect on scrapie activity. Concentrated preparations of sufficient potency for characterization of virus particles by electron microscopy were not obtained. From various experimental evidence the scrapie agent, if a virus, appears to be of small size. The strong association of the agent with tissue debris suggests that the presence of a tissue component may be necessary in the experimental transmission of the condition.

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/content/journal/micro/10.1099/00221287-40-1-71
1965-07-01
2024-12-03
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References

  1. Chandler R. L. 1963; Experimental scrapie in the mouse. Res. Vet. Sci 4:276
    [Google Scholar]
  2. Eichberg Jun J., Whittaker V. P., Dawson R. M. C. 1964; Distribution of lipids in subcellular particles of guinea-pig brain. Biochem. J 92:91
    [Google Scholar]
  3. Hunter G. D., Millson G. C. 1965; Studies on the heat stability and chromato-graphic behaviour of the scrapie agent. J. gen. Microbiol 37:251
    [Google Scholar]
  4. Hunter G. D., Millson G. C., Chandler R. L. 1963; Observations on the comparative infectivity of cellular fractions derived from homogenates of mouse-scrapie brain. Res. Vet. Sci 4:543
    [Google Scholar]
  5. Hunter G. D., Millson G. C., Meek G. 1964; The intracellular location of the agent of mouse scrapie. J. gen. Microbiol 34:319
    [Google Scholar]
  6. Johnson M. K. 1962; Treatment of subcellular particles prior to assay of the enzymatic marker, succinic dehydrogenase. Nature, Londs 196:1210
    [Google Scholar]
  7. Mould D. L., Dawson A. McL., Smith W. 1964; The infectivities of brain-cell fractions from mice infected with Suffolk sheep scrapie agent. Biochem. J 91:13
    [Google Scholar]
  8. Mould D. L., Dawson A. McL., Smith W. 1965; Serapie in mice. The stability of the agent to various suspending media, pH and solvent extraction. Res. Vet. Sci 6:151
    [Google Scholar]
  9. Mould D. L., Smith W. 1962; The causal agent of serapie. I. The extraction of the agent from infected sheep tissue. J. camp. Path 72:97
    [Google Scholar]
  10. Mould D. L., Smith W., Dawson A. McL. 1964; The elution behaviour of serapie brain tissue through calcium phosphate columns. J. gen. Microbiol 35:491
    [Google Scholar]
  11. Pattison I. H., Millson G. C. 1962; Distribution of the serapie agent in the tissues of experimentally inoculated goats. J. comp. Path 72:233
    [Google Scholar]
  12. Pattison I. H., Sansom B. F. 1964; Dialysis of the serapie agent. Res. Vet. Sci 5:340
    [Google Scholar]
  13. Slater J. S. 1965; Succinic dehydrogenase, cytochrome oxidase and acid phosphatase activities in the brains of scrapie-infected goats and mice. Res. Vet. Sci 6:155
    [Google Scholar]
  14. Stamp J. T., Brotherston J. G., Zlotnik I., Mackay J. M. K., Smith W. 1959; Further studies on serapie. J. comp. Path 69:268
    [Google Scholar]
  15. Zlotnik I., Rennie J. C. 1962; The pathology of the brain of mice inoculated with tissues from serapie sheep. J. comp. Path 72:360
    [Google Scholar]
  16. Zlotnik I., Rennie J. C. 1963; Further observations on the experimental transmission of serapie from sheep and goats to laboratory mice. J. comp. Path 73:150
    [Google Scholar]
  17. Zlotnik I., Rennie J. C. 1965; The experimental transmission of mouse passaged serapie to goats, sheep, rats and hamsters. J. comp. Path
    [Google Scholar]
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