1887

Abstract

SUMMARY

Sensitivity to ampicillin and cephalothin, the formation and induction of penicillinase and cephalosporinase, and the role of cephalosporinase in resistance to cephalothin were investigated in nine strains of Gramnegative bacteria. Naturally occurring strains resistant to cephalothin formed cephalosporinase, and ampicillin-resistant strains formed penicillinase. Cephalothin-resistant strains were all resistant to ampicillin, but the ampicillin-resistant strain of was sensitive to cephalothin and did not form cephalosporinase. Penicillinase and cephalosporinase activity were induced by benzylpenicillin in five strains of Gram-negative bacteria and cephalosporinase was also induced by cephalothin and 6-amino-penicillanic acid in four of the inducible strains examined. Methicillin and quinacillin were variable in effect as inducers and cloxacillin partially inhibited cephalosporinase activity of two strains. Intrinsic resistance to cephalothin was high in four of five naturally occurring resistant strains.

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/content/journal/micro/10.1099/00221287-40-1-119
1965-07-01
2021-07-31
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References

  1. Ayliffe G. A. J. 1963; Ampicillin inactivation and sensitivity of coliform bacilli. J. gen. MicroUol 30:339
    [Google Scholar]
  2. Ayliffe G. A. J. 1964; Induction of cephalosporinase and penicillinase in Proteus species. Nature, Lond 201:1032
    [Google Scholar]
  3. Barber M., Waterworth P. M. 1964; Penicillinase resistant penicillins and cephalo-sporins. Brit. med. J. ii344
    [Google Scholar]
  4. Chauvette R. R., Flynn E. H., Jackson B. G., Lavagnino E. R., Morin R. B., Mueller R. A., Pioch R. P., Roeske R. W., Ryan C. W., Spencer J. L., Van Heyningen E. 1962; Chemistry of the cephalosporin antibiotics, ii. Preparation of a new class of antibiotics and the relation of structure to activity. J. Am. Chem. Soc 84:3401
    [Google Scholar]
  5. Cowan S. T., Steel K. J. 1961; Diagnostic tables for the common medical bacteria. J. Hyg., Camb 59:357
    [Google Scholar]
  6. Crompton B., Jago M., Crawford K., Newton G. G. F., Abraham E. P. 1962; Behaviour of some derivatives of 7-aminocephalosporanic acid and 6-aminopenicillanic acid as substrates, inhibitors and inducers of penicillinases. Biochem. J 83:52
    [Google Scholar]
  7. Fleming P. C, Goldner M., Glass D. G. 1963; Observations on the nature, distribution and significance of cephalosporinase. Lancet, i1399
    [Google Scholar]
  8. Hamilton-Miller J. M. T. 1963; Inducible penicillinase in Proteus morganii . Biochem. Biophys. Res. Comm 13:43
    [Google Scholar]
  9. Hamilton-Miller J. M. T., Smith J. T. 1964; Inhibition of penicillinase from Gram-positive and Gram-negative bacteria by substrate analogues. Nature, Lond 201:999
    [Google Scholar]
  10. Jago M., Migliacci A., Abraham E. P. 1963; Production of a cephalosporinase by Pseudomonas pyocyanea . Nature, Lond 199:375
    [Google Scholar]
  11. Muggleton P. W., O’Callaghan C. H., Stevens W. K. 1964; Laboratory evaluation of a new antibiotic—cephaloridine (Ceporin). Brit. med. J. ii1234
    [Google Scholar]
  12. Percival A., Brumfitt W., de Louvois J. 1963; The role of penicillinase in determining natural and acquired resistance of Gram-negative bacteria to penicillins. J. gen. MicroUol 32:77
    [Google Scholar]
  13. Perret C. J. 1954; Iodometric assay of penicillinase. Nature, Lond 174:1012
    [Google Scholar]
  14. Richards H. C, Housley J. R., Spooner D. F. 1963; Quinacillin: A new penicillin with unusual properties. Nature, Lond 199:354
    [Google Scholar]
  15. Smith J. T., Hamilton-Miller J. M. T. 1963; Difference between penicillinases from Gram-positive and Gram-negative bacteria. Nature, Lond 197:976
    [Google Scholar]
  16. Smith J. T., Hamilton-Miller J. M. T., Knox R. 1962; Isoxazolyl penicillins and penicillinase. Nature, Lond 195:1300
    [Google Scholar]
  17. Sutherland R. 1964; The nature of the insensitivity of Gram-negative bacteria towards penicillins. J. gen. Microbiol 34:85
    [Google Scholar]
  18. Sutherland R., Batchelor F. R. 1964; Synergistic activity of penicillins against penicillinase-producing Gram-negative bacilli. Nature, Lond 201:868
    [Google Scholar]
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