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Volume 4, Issue 3

Identification of Characteristic Extracellular Ninhydrin-Positive Substances Produced by some Bacteria Free

Abstract

SUMMARY: When certain species of bacteria are grown in an acid-hydrolysate of casein medium, ninhydrin-positive substances which were not present in the un-inoculated medium appear on chromatograms of the culture filtrates. and produce -aminobutyric acid by decarboxy-lation of glutamic acid. The substance produced by resists acid hydrolysis, gives a positive Sakaguchi reaction and matches arginine on two-dimensional chromatography. The substances produced by and , but not by any of the other species of Clostridia examined, are -aminopentanoic acid, probably derived from proline, and α- and -aminobutyric acids produced by unknown mechanisms. and each produce two polypeptides containing a high proportion of amino-acids in the groups valine/methionine and leucine/leucine. These two pairs of polypeptides are similar in value and gross amino-acid composition. produces α-aminobutyric acid which may be derived from threonine.

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© Society for General Microbiology 1950
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/content/journal/micro/10.1099/00221287-4-3-501
1950-09-01
2024-12-05
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/content/journal/micro/10.1099/00221287-4-3-501
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Identification of Characteristic Extracellular Ninhydrin-Positive Substances Produced by some Bacteria
Microbiology 4, 501 (1950); https://doi.org/10.1099/00221287-4-3-501
/content/journal/micro/10.1099/00221287-4-3-501
/content/journal/micro/10.1099/00221287-4-3-501
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