SUMMARY: When certain species of bacteria are grown in an acid-hydrolysate of casein medium, ninhydrin-positive substances which were not present in the un-inoculated medium appear on chromatograms of the culture filtrates. and produce γ-aminobutyric acid by decarboxy-lation of glutamic acid. The substance produced by resists acid hydrolysis, gives a positive Sakaguchi reaction and matches arginine on two-dimensional chromatography. The substances produced by and , but not by any of the other species of Clostridia examined, are δ-aminopentanoic acid, probably derived from proline, and α- and γ-aminobutyric acids produced by unknown mechanisms. and each produce two polypeptides containing a high proportion of amino-acids in the groups valine/methionine and leucine/leucine. These two pairs of polypeptides are similar in value and gross amino-acid composition. produces α-aminobutyric acid which may be derived from threonine.


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