1887

Abstract

SUMMARY: The method used in maintaining cultures of affects the relationship between duration of lag phase and age of inoculum in a glucose ammonium salt medium. Organisms kept in broth may show pronounced early lag whilst those from agar slopes do not. Previous subcultivation with aeration decreases the lag. Bacteria showing no lag may do so when they have been washed; glutamate, α-ketoglutarate and succinate partially remove the lag so produced, whilst malate, fumarate and aspartate do not.

The initial growth rate of light inocula showing no lag is increased by the addition of glutamate, aspartate, α-ketoglutarate, succinate and oxalacetate; but pyruvate, fumarate and malate have no action or are slightly inhibitory. The effect of these compounds over a concentration range of 0 to 1·6 × 10 g. mol./l. was studied quantitatively. Filtrates from growing cultures have a similar effect on initial growth rate; those taken during the late logarithmic phase are more effective than those taken earlier. The effects of both filtrates and added compounds are confined to initial growth; the rate of growth in the later phase, when turbidity becomes visible, remains unaltered. This behaviour is dependent on the amount of O available in the medium.

Early lag is a phase of greatly reduced rate of growth rather than complete absence of cell division. Glutamate and other effective compounds increase the rate to values approaching normal.

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1950-09-01
2021-08-04
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References

  1. Ajl S.J., Werkman C.H. 1948; Replacement of CO2 in heterotrophic metabolism. Arch. Biochem 19:483
    [Google Scholar]
  2. Ajl S.J., Werkman C.H. 1949; Anaerobic replacement of carbon dioxide. Proc. Soc. exp. Biol.,N.Y 70:522
    [Google Scholar]
  3. Buffa P., Coxon R.V., Liébecq C., Peters R.A. 1949; Experiments on the tricarboxylic acid cycle in brain and kidney tissues. 1st Internal. Congr. Biochem. Abstr52/3 p. 570
    [Google Scholar]
  4. Dagley S., Dawes E.A., Morrison G.A. 1949a; Application of bacterial kinetics to biochemical problems. Nature, Lond 163:532
    [Google Scholar]
  5. Dagley S., Dawes E.A., Morrison G.A. 1949b; Influence of amino-acids, and compounds in the Krebs oxidation cycle, on ‘early lag’. Biochem. J 45:xxvi
    [Google Scholar]
  6. Fenton H.J.H., Jones H.O. 1900; The oxidation of organic acids in presence of ferrous iron. Part I. J. chem. Soc 77:69
    [Google Scholar]
  7. Hewitt L.F. 1936 Oxidation reduction potentials in bacteriology and biochemistry, 4th ed.. London: London County Council;
    [Google Scholar]
  8. Hinshelwood C.N. 1946 The chemical kinetics of the bacterial cell Oxford University Press;
    [Google Scholar]
  9. Lodge R.M., Hinshelwood C.N. 1939; Physicochemical aspects of bacterial growth. Part IV. Conditions determining stationary populations and growth rates of Bact. Lactis Aerogenes in synthetic media. J. chem. Soc p. 1683
    [Google Scholar]
  10. Lodge R.M., Hinshelwood C.N. 1943a; Physicochemical aspects of bacterial growth. Part VIII. Growth of Bact. Lactis Aerogenes in media containing ammonium sulphate or various amino acids. J. chem. Soc p. 208
    [Google Scholar]
  11. Lodge R.M., Hinshelwood C.N. 1943b; Physicochemical aspects of bacterial growth, Part IX. The lag phase of Bact. Lactis Aerogenes . J. chem. Soc p. 213
    [Google Scholar]
  12. Lwoff A., Monod J. 1947; Essai d’analyse du role de Panhydride carbonique dans la croissance microbienne. Ann. Inst. Pasteur 73:323
    [Google Scholar]
  13. Monod J. 1942 Recherches sur la croissance des cultures bactériennes Paris: Herman & Cie;
    [Google Scholar]
  14. Morrison G.A., Hinshelwood C.N. 1949; Nitrogen utilization and growth of coliform bacteria. Part III. Nitrogen utilization and lag phase. J. chem. Soc p. 380
    [Google Scholar]
  15. O’Meara R.A.Q., Mcnally P.A., Nelson H.G. 1947; The intra-cellular mode of action of the sulphonamide derivatives. Lancet ii:747
    [Google Scholar]
  16. Poole A., Hinshelwood C.N. 1940; Physicochemical aspects of bacterial growth. Part VI. The influence of toxic substances on growth rate, stationary population and fermentation reactions of Bact. Lactis Aerogenes . J. chem. Soc p. 1565
    [Google Scholar]
  17. Roine P. 1947; On the formation of primary amino acids in the protein synthesis in yeast. Ann. Acad. Sci. Fennicae, A 11:26
    [Google Scholar]
  18. Winslow C.-E.A., Walker H.H., Sutermeister M. 1932; The influence of aeration and of sodium chloride upon the growth curve of bacteria in various media. J. Bact 24:185
    [Google Scholar]
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