Anaerobic and Aerobic Growth of Purple Bacteria (Athiorhodaceae) in Chemically Defined Media Free

Abstract

SUMMARY: Studies on the anaerobic growth of aerobically adapted purple bacteria show that the same growth factors suffice for both aerobic and anaerobic growth, thus supporting van Niel's formulations of photosynthesis and carbon assimilation in these forms, and his thesis that the previously observed need for peptone or yeast extract could be attributed to their content of essential growth factors. Combinations of glutamate and succinate (or fumarate) were especially good promoters of growth in malate media; it is possible that their effectiveness was referable to their allowing a by-pass of the CO requirement. The production of molecular hydrogen by purple bacteria is discussed in relation to the reducing intensities attained in cultures.

A bottle technique for anaerobic cultures, and a flask technique for aerobic cultures, are described in detail.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-4-3-286
1950-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/4/3/mic-4-3-286.html?itemId=/content/journal/micro/10.1099/00221287-4-3-286&mimeType=html&fmt=ahah

References

  1. Ajl S.J., Werkman C.H. 1949; Anaerobic replacement of carbon dioxide. Proc. Soc. exp. Biol., N.Y. 70:522
    [Google Scholar]
  2. Gest H., Kamen M.D. 1949a; Photoproduction of molecular hydrogen by Rhodospirillum rubrum. . Science 109:558
    [Google Scholar]
  3. Gest H., Kamen M.D. 1949b; Studies on the metabolism of photosynthetic bacteria. IV. Photochemical production of molecular hydrogen by growing cultures of photosynthetic bacteria. J. Bact. 58:239
    [Google Scholar]
  4. Giesberger G. 1947; Some observations on the culture, physiology and morphology of some brown-red Rhodospirillum, species. Antonie v. Leeuwenhoek J. Microbiol. Serol. 13:185
    [Google Scholar]
  5. Hewitt L.F. 1948 Oxidation-reduction potentials in bacteriology and biochemistry, 5th ed.. London: Staples Press Ltd; Agents, London County Council
    [Google Scholar]
  6. Hutner S.H. 1946; Organic growth essentials of the aerobic nonsulfur photosynthetic bacteria. J. Bact. 52:213
    [Google Scholar]
  7. Hutner S.H. 1948; Essentiality of constituents of sea water for growth of a marine diatom. Trans. N.Y. Acad. Sci. Ser. II:10–136
    [Google Scholar]
  8. Hutner S.H., Bjerknes Clara A. 1948; Volatile preservatives for culture media. Proc. Soc. exp. Biol., N.Y. 67:281
    [Google Scholar]
  9. Hutner S.H., Provasoli L., Stokstad E.L.R., Hoffmann C.E., Belt M., Franklin A.L., Jukes T.H. 1949; Assay of anti-pernicious ANEMIA factor with Euglena . Proc. Soc. exp. Biol., N.Y. 70:118
    [Google Scholar]
  10. Lwoff A., Monod J. 1947; Essai d’analyse du rôle de l’anhydride carbonique dans la croissance microbienne. Ann. Inst. Pasteur 73:328
    [Google Scholar]
  11. Michaelis L., Hill E.S. 1933; The viologen indicators. J. gen. Physiol. 16:859
    [Google Scholar]
  12. Muller F.M. 1933; On the metabolism of the purple sulphur bacteria in organic media. Arch. Mikrobiol. 4:131
    [Google Scholar]
  13. Niel C.B.Van. 1931; On the morphology and physiology of the purple and green sulphur bacteria. Arch. Mikrobiol. 3:1
    [Google Scholar]
  14. Niel C.B.Van. 1936; On the metabolism of the Thiorhodaceae. Arch. Mikrobiol. 7:32
    [Google Scholar]
  15. Niel C.B.Van. 1944; The culture, general physiology, morphology, and classification of the non-sulfur purple and brown bacteria. Bact. Rev. 8:1
    [Google Scholar]
  16. Niel C.B.Van. 1947; Studies on the pigments of the purple bacteria. III. The yellow and red pigments of Rhodopseudomonas spheroides. . Antonie v. Leeuwenhoek J. Microbiol. Serol. 12:156
    [Google Scholar]
  17. Niel C.B.Van. 1949; The comparative biochemistry of photosynthesis. In Photosynthesis in plants p. 437, ed.. Frank J., Loomis W.E. Ames, Iowa: Iowa State College Press;
    [Google Scholar]
  18. Reed G.B., Orr J.H. 1943; Cultivation of anaerobes and oxidation-reduction potentials. J.Bact. 45:309
    [Google Scholar]
  19. Richardson G.M. 1036; The nutrition of Staphylococcus aureus. Necessity for uracil in anaerobic growth. Biochem. J. 30:2184
    [Google Scholar]
  20. Roelofsen P.A. 1935; On the metabolism of the purple sulphur bacteria. Proc. Acad. Sci. Amst. 37:660
    [Google Scholar]
  21. Welch A.D. 1949; Mechanism of the growth-promoting effect of ascorbic acid on Lactobacillus Icickmanii and the reduction of oxidation products of vitamin B13 . Arch. Biochem. 22:486
    [Google Scholar]
  22. Wynne E.S., Foster J.K. 1948; Physiological studies on spore germination, with special reference to Clostridium botulinum. III. Carbon dioxide and germination, with a note on carbon dioxide and aerobic spores. J. Bact. 55:331
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-4-3-286
Loading
/content/journal/micro/10.1099/00221287-4-3-286
Loading

Data & Media loading...

Most cited Most Cited RSS feed