1887

Abstract

SUMMARY: The power of fermentation by yeast cells was decreased when the cultures were grown in media deficient in any of seven essential growth factors. A rapid increase in the rate of fermentation upon the addition of a growth factor was obtained in every case except that of inositol, where the response was much slower. The absence of a nitrogen source decreased the response to biotin, pantothenic acid or pyridoxin to a much greater extent than to aneurin or nicotinic acid. Differences were noted also in the behaviour of the two groups towards sodium azide, which can be explained on the assumption that biotin, pantothenic acid and pyridoxin affect fermentation only in a relatively indirect manner.

The decrease in the power of fermentation produced by pyridoxin deficiency was not observed until the pyridoxin content of the cells was only about one-fifth of the normal. Such yeast showed also an enhanced Pasteur effect. Pyridoxin deficiency produced no decrease in the proportion of glucose assimilated by resting cells.

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/content/journal/micro/10.1099/00221287-4-2-171
1950-05-01
2021-10-22
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References

  1. Atkin L., Schultz A. S., Frey C. N. 1946; Enzymes and their Role in Wheat Technology. Anderson J. A. ed Chap. xi New York: Interscience Publishers Inc;
    [Google Scholar]
  2. Brockmann M. C., Stier T. J. B. 1947; Cellular mechanisms controlling rates of glucose consumption by yeast. J. cell. comp. Physiol 29:159
    [Google Scholar]
  3. Burkholder P. R., McVeigh J., Moyer D. 1944; Studies on some growth factors of yeasts. J. Bact 48:385
    [Google Scholar]
  4. Clifton C. E. 1987; On the possibility of preventing assimilation in respiring cells. Enzymologia 4:246
    [Google Scholar]
  5. Clifton C. E. 1946; Microbial assimilations. Advances in Enzymology 6:269
    [Google Scholar]
  6. Dixon M. 1943 Manometric Methods, 2nd. ed. p. 7 Cambridge: University Press ;
    [Google Scholar]
  7. Ehkenfest E., Ronzoni E. 1933; Effect of dinitrophenol on oxidation of tissues. Proc. Soc. exp. Biol., N.Y 31:318
    [Google Scholar]
  8. Emery W. B., Mcleod N., Robinson F. A. 1946; Comparative microbiological assays of members of the vitamin B complex in yeast and liver extracts. Biochem. J 40:426
    [Google Scholar]
  9. Hopkins R. H., Pennington R. J. 1947; The assay of the vitamin B6 complex. Biochem. J 41:110
    [Google Scholar]
  10. Lipmann F. 1941; Metabolic generation and utilization of phosphate bond energy. Advances in Enzymology 1:99
    [Google Scholar]
  11. Rainbow C. 1948; p-Aminobenzoic acid a growth-factor for certain brewer’s yeasts. Nature; Lond: 162572
    [Google Scholar]
  12. Rothstein A. 1946; Phosphorylation in living yeast cells. J. cell. comp. Physiol 28:221
    [Google Scholar]
  13. Spiegelman S. 1946; The inhibition of enzyme formation. Fed. Proc 5:99
    [Google Scholar]
  14. Spiegelman S., Kamen M. D. 1946; The site of uncoupling of phosphorylation from carbohydrate metabolism in the presence of NaN3. Fed. Proc 5:99
    [Google Scholar]
  15. Spiegelman S., Kamen M. D., Dunn R. 1946; Mechanism of azide inhibition of synthetic activity and its relation to phosphorylation. Fed. Proc 5:99
    [Google Scholar]
  16. Thorne R. S. W. 1939; Mathematical representation of the course of yeast growth. J. Inst. Brew 45:472
    [Google Scholar]
  17. Williams R. J., Mosher W. A., Rohrman E. 1986; The importance of ‘pantothenic acid’ in fermentation, respiration and glycogen storage. Biochem. J 30: 2086
    [Google Scholar]
  18. Winzler R. J. 1944; Azide inhibition of anaerobic assimilation of glucose by yeast and its application to the determination of fermentable sugar. Science 99:327
    [Google Scholar]
  19. Winzler R. J., Burk D., du Vigneaud V. 1944; Biotin in fermentation, respiration, growth and nitrogen assimilation by yeast. Arch. Biochem 5:25
    [Google Scholar]
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