1887

Abstract

SUMMARY: Strains of Group A streptococci produced an extracellular carbohydrase which degraded starch. The enzyme in crude undialysed culture filtrates bore a general resemblance to pancreatic diastase. Amylolytic activity was decreased in environmental conditions which also affected streptococcal proteinase, namely in cultures grown at 23°, in filtered broth, or after serial mouse passage. Amylase production was associated with hyaluronidase production in strains having the type-specific M antigen 4, or the non-specific T antigen 4, whereas proteinase-positive strains of the same group of closely related specific Types 4, 24, 26, 28, 29, 46 were usually amylase-negative; but the association between production of any two of the three enzymes was not complete. Hyaluronidase-positive Type 22 strains did not produce amylase, and with two exceptions, a Type 2 strain and a strain serologically unidentified, other representative serological types were amylase-negative, though some strains degraded starch in growing cultures.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-4-2-156
1950-05-01
2022-01-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/4/2/mic-4-2-156.html?itemId=/content/journal/micro/10.1099/00221287-4-2-156&mimeType=html&fmt=ahah

References

  1. Andrewes F. W. 1930; Note on the fermentation of starch by certain haemolytic streptococci. J. Path. Bact 33:145
    [Google Scholar]
  2. Baldwin E. 1948 Dynamic Aspects of Biochemistry, 2nd ed. Cambridge University Press;
    [Google Scholar]
  3. Butt E. M., Bonynge C. W., Joyce R. L. 1986; The demonstration of capsules about haemolytic streptococci with india ink or Azo blue. J. infect. Dis 58:5
    [Google Scholar]
  4. Crowley N. 1944; Hyaluronidase production by haemolytic streptococci of human origin. J. Path. Bact 56:27
    [Google Scholar]
  5. Dole V. P. 1946; A dialysate medium for the culture of Group A streptococci. Proc. Soc. exp. Biol., N.Y 63:122
    [Google Scholar]
  6. Elliott S. D. 1943; Type relationships among Group A streptococci. Brit. J. exp. Path 24:159
    [Google Scholar]
  7. Elliott S. D. 1945; A proteolytic enzyme produced by Group A streptococci with special reference to its effect on the type specific M antigen. J. exp. Med 81:578
    [Google Scholar]
  8. Elliott S. D., Dole V. P. 1947; An inactive precursor of streptococcal proteinase. J. exp. Med 85:305
    [Google Scholar]
  9. Griffith F. 1934; The serological classification of streptococcus pyogenes. J. Hyg., Camb 34:542
    [Google Scholar]
  10. Keogh E. V., Simmons R. T. 1940; Cultural methods as an aid in type differentiation of Group A haemolytic streptococci. J. Path. Bact 50:137
    [Google Scholar]
  11. Lancefield R. C. 1940; Type specific antigens, M and T, or matt and glossy variants of Group A streptococci. J. exp. Med 71:539
    [Google Scholar]
  12. Lancefield R. C. 1940–1; Specific relationship of cell composition to biological activity of haemolytic streptococci. Harvey Lect 36:251
    [Google Scholar]
  13. Lancefield R. C. 1943; Studies of the antigenic composition of Group A streptococci. J. exp. Med 78:465
    [Google Scholar]
  14. Lancefield R. C., Dole V. P. 1946; The properties of T antigens extracted from Group A streptococci. J. exp. Med 84:449
    [Google Scholar]
  15. Maxted W. R. 1948; Preparation of streptococcal extracts for Lancefield grouping. Lancet ii:255
    [Google Scholar]
  16. Mccarty M. 1948; The occurrence of nucleases in culture filtrates of Group A streptococci. J. exp. Med 88:181
    [Google Scholar]
  17. Mcclean D. 1942; The in vivo decapsulation of streptococci by hyaluronidase. J. Path. Bact 54:284
    [Google Scholar]
  18. Mcclean D., Hale C. W. 1941; Studies on diffusing factors. Biochem. J 35:159
    [Google Scholar]
  19. Meyer K. 1947; The biological significance of hyaluronic acid and hyaluronidase. Physiol. Rev 27:3
    [Google Scholar]
  20. Miles A. A., Misra S. S. 1938; The estimation of the bactericidal powers of the blood. J. Hyg., Camb 38:782
    [Google Scholar]
  21. Phillips R. A., Van Slyke D. D., Dole V. P., Emerson K. Jr Hamilton P. B., Archibald R. M. 1945; Copper sulphate method for measuring specific gravities of whole blood and plasma. Indian med. Gaz 80:275
    [Google Scholar]
  22. Pike R. M. 1948; Studies on streptococcal hyaluronic acid and hyaluronidase. J. infect. Dis 83:1
    [Google Scholar]
  23. Robertson W. van. B, Ropes M. W., Bauer W. 1940; Mucinase: A bacterial enzyme which hydrolyses synovial fluid mucin and other mucins. J. biol. Chem 133:261
    [Google Scholar]
  24. Rogers H. J. 1944; A simplified culture medium for the production and purification of hyaluronidase. J. Path. Bact 66:284
    [Google Scholar]
  25. Rogers H. J. 1948; The complexity of bacterial hyaluronidases. Biochem. J 42:663
    [Google Scholar]
  26. Sherman J. 1937; The streptococci. Bact. Rev 1:3
    [Google Scholar]
  27. Swift H., Wilson A., Lancefield R. C. 1943; The typing of Group A streptococci by M precipitin reactions in capillary pipettes. J. exp. Med 78:127
    [Google Scholar]
  28. Todd E. W., Hewitt L. 1932; A new culture medium for the production of antigenic haemolysin. J. Path. Bact 35:973
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-4-2-156
Loading
/content/journal/micro/10.1099/00221287-4-2-156
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error