SUMMARY: Paper chromatography was used for studying qualitatively and quantitatively the reducing sugars liberated from sucrose during the synthesis of polysaccharides by certain bacteria. Glucose and fructose were the only sugars detected, varying in amount with the bacteria and with the type of polysaccharide synthesized. The results suggest that both polymerizing and hydrolytic enzymes were present.
CooperE. A.,
DakerW. D.,
StaceyM.1938; Enzyme formation and polysaccharide synthesis by bacteria. III. Polysaccharides produced by ‘nitrogen fixing’ organisms. Biochem. J 32:1752
GenghofD. S.,
HehreE. J.,
NeillJ. M.1946; Serological reactions of levans formed from sucrose and raffinose by certain bacilli. Proc. Soc. exp. Biol., N.Y 61:339
HehreE. J.,
SuggG.1942; Serologically reactive polysaccharides produced through the action of bacterial enzymes. I. Dextran of Leuconostoc mesen-teroides from sucrose. J. exp. Med 75:339
PartridgeS. M.1948; Filter-paper partition chromatography of sugars. I. General description and application to the qualitative analyses of sugars in apple juice, egg white and foetal blood of sheep. Biochem. J 42:238
TarrA.,
HibbertH.1931; Studies on reactions relating to carbohydrate polysaccharides. XXXVII. The formation of dextran by Leuconostoc mesenteroides. Canad. J. Res 5:414