1887

Abstract

SUMMARY: Paper chromatography was used for studying qualitatively and quantitatively the reducing sugars liberated from sucrose during the synthesis of polysaccharides by certain bacteria. Glucose and fructose were the only sugars detected, varying in amount with the bacteria and with the type of polysaccharide synthesized. The results suggest that both polymerizing and hydrolytic enzymes were present.

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/content/journal/micro/10.1099/00221287-4-1-87
1950-01-01
2022-05-16
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References

  1. Cooper E. A. 1939; Polysaccharide synthesis by the bacilli. J. Soc. chem. Ind., Lond 58:T229
    [Google Scholar]
  2. Cooper E. A., Carruthers A. 1936; Enzyme formation and polysaccharide synthesis by bacteria. II. Biochem. J 30:1001
    [Google Scholar]
  3. Cooper E. A., Daker W. D., Stacey M. 1938; Enzyme formation and polysaccharide synthesis by bacteria. III. Polysaccharides produced by ‘nitrogen fixing’ organisms. Biochem. J 32:1752
    [Google Scholar]
  4. Cooper E. A., Perston J. F. 1935; Enzyme formation and polysaccharide synthesis by bacteria. Biochem. J 29:2267
    [Google Scholar]
  5. Flood A. E., Hirst E. L., Jones J. K. N. 1947; Quantitative estimation of mixtures of sugars by the paper chromatogram method. Nature; Lond: 16086
    [Google Scholar]
  6. Forsyth W. G. C. 1948; Colour reagents for the paper chromatography of sugars. Nature; Lond: 161239
    [Google Scholar]
  7. Forsyth W. G. C., Webley D. M. 1948; A method for studying the carbohydrate metabolism of micro-organisms. Nature; Lond: 162150
    [Google Scholar]
  8. Forsyth W. G. C., Webley D. M. 1949; Polysaccharides synthesized by aerobic mesophilic spore-forming bacteria. Biochem. J 44:455
    [Google Scholar]
  9. Genghof D. S., Hehre E. J., Neill J. M. 1946; Serological reactions of levans formed from sucrose and raffinose by certain bacilli. Proc. Soc. exp. Biol., N.Y 61:339
    [Google Scholar]
  10. Hehre E. J. 1941; Production from sucrose of a serologically reactive polysaccharide by a sterile bacterial extract. Science 93:237
    [Google Scholar]
  11. Hehre E. J. 1946; The enzymatic synthesis of dextran. J. biol. Chem 163:221
    [Google Scholar]
  12. Hehre E. J., Sugg G. 1942; Serologically reactive polysaccharides produced through the action of bacterial enzymes. I. Dextran of Leuconostoc mesen-teroides from sucrose. J. exp. Med 75:339
    [Google Scholar]
  13. Hestrin S., Avineri-Shapiro S. 1944; The mechanism of polysaccharide production from sucrose. Biochem. J 38:2
    [Google Scholar]
  14. Partridge S. M. 1946; Application of the paper partition chromatogram to the qualitative analysis of reducing agars. Nature; Lond: 158270
    [Google Scholar]
  15. Partridge S. M. 1948; Filter-paper partition chromatography of sugars. I. General description and application to the qualitative analyses of sugars in apple juice, egg white and foetal blood of sheep. Biochem. J 42:238
    [Google Scholar]
  16. Somogyi M. 1945; New reagent for the determination of sugars. J. biol. Chem61
    [Google Scholar]
  17. Tarr A., Hibbert H. 1931; Studies on reactions relating to carbohydrate polysaccharides. XXXVII. The formation of dextran by Leuconostoc mesenteroides. Canad. J. Res 5:414
    [Google Scholar]
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