1887

Abstract

SUMMARY: The effect of dilution on numbers of local lesions produced by plant viruses was tested graphically and statistically for compatibility with contrasting hypotheses. Experimental results are incompatible with the hypothesis that lesions are produced because of chance encounters between single virus particles and susceptible regions of a uniform type, and that variations in response to similar inoculation between different leaves or plants occur solely because of variations in numbers of such regions. The results are compatible with the hypothesis that individual susceptible regions vary in susceptibility so that different doses of virus are needed to produce a lesion (the variations being such that the logarithms of minimal effective doses are normally distributed). The second hypothesis is, therefore, more probably correct, but the first can be fitted to experimental results by introducing various auxiliary assumptions, such as the existence of qualitatively different virus particles and susceptible regions. Conclusive evidence is unlikely to come from mathematical treatment of existing results, but only from a new experimental approach.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-4-1-53
1950-01-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/4/1/mic-4-1-53.html?itemId=/content/journal/micro/10.1099/00221287-4-1-53&mimeType=html&fmt=ahah

References

  1. Baud J. G. 1937a; The use of numbers of lesions for comparing the concentrations of plant virus, suspensions. I. Dilution experiments with purified suspensions. Ann. appl. Biol 24:88
    [Google Scholar]
  2. Bald J. G. 1937b; The use of numbers of lesions for comparing the concentrations of plant virus suspensions. 11. Distortion of the dilution series. Ann. appl. Biol 24:56
    [Google Scholar]
  3. Bald J. G. 1937c; The use of numbers of lesions for comparing the concentrations of plant virus suspensions. IV. Modification of the simple dilution equation. Austr. J. exp. Biol. med. Sci 15:211
    [Google Scholar]
  4. Bawden F. C., Pirie N. W. 1945; The isolation and properties of tobacco mosaic virus in different states of aggregation. Brit. J. exp. Path 26:294
    [Google Scholar]
  5. Bliss C. I. 1935; The calculation of the dosage-mortality curve. Ann. appl. Biol 22:134
    [Google Scholar]
  6. Bryan W. R., Beard J. W. 1940; Host influence on the characterization of response to the papilloma protein and to vaccinia virus. J. infect. Dis 67:5
    [Google Scholar]
  7. Crook E. M., Sheffield F. M. L. 1946; State of aggregation of tobacco mosaic virus. Brit. J. exp. Path 27:328
    [Google Scholar]
  8. Finney D. J. 1947 Probit Analysis Cambridge University Press;
    [Google Scholar]
  9. Gaddum J. H. 1933; Methods of biological assay depending on a quantal response. Spec. Hep. Ser. Med. Res. Coun183 London: H. M. Stationery Office;
    [Google Scholar]
  10. Haldane J. B. S. 1939; Sampling errors in the determination of virus density by the dilution method. J. Hyg., Camb 39:289
    [Google Scholar]
  11. Kleczkowski A. 1949; The transformation of local lesion counts for statistical analysis. Ann. appl. Biol 36:139
    [Google Scholar]
  12. Lauffer M. A., Price W. C. 1945; Infection by viruses. Arch. Biochem 8:449
    [Google Scholar]
  13. Parker R. F. 1938; Statistical studies of the nature of the infectious unit of vaccinia virus. J. exp. Med 67:725
    [Google Scholar]
  14. Sadasivan T. S. 1940; A quantitative study of the interaction of viruses in plants. Ann. appl. Biol 27:359
    [Google Scholar]
  15. Youden W. J., Beale H. P., Guthrie J. D. 1935; Relation of virus concentration to the numbers of lesions produced. Contr. Boyce Thompson Inst 7:37
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-4-1-53
Loading
/content/journal/micro/10.1099/00221287-4-1-53
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error