SUMMARY: Cysts of two amoebae from soil were grown in single-cell cultures to test the effect upon excystment of the presence or absence of bacteria, age and type of bacteria, age of cyst, and the concentration of sodium chloride. Within the limits of the experiments excystment in one species was independent of the presence of bacteria, and unaffected by their age and type or by the age of cyst. The other species was more sensitive and could not excyst without living bacteria of a suitable type, and the cysts become less likely to develop with age. Excystment in both species was adversely affected by increasing the concentration of sodium chloride, distilled water giving the best results.


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