1887

Abstract

SUMMARY

The influence of Mg-limitation, compared with carbon-limitation, on bacterial concentration, and on protein, carbohydrate, RNA and DNA contents of cultures (grown in the chemostat at several dilution rates) was determined. In both types of culture the bacterial protein, carbohydrate and DNA contents varied slightly, and the RNA content grossly, with changes in dilution rate. Bacterial yield varied with growth rate, and to a marked degree in the Mg-limited culture; this resulted from Mg control of RNA synthesis. A growth-rate independent stoichiometry between RNA and Mg was observed; 4 moles of RNA nucleotide were synthesized per mole of Mg present in the culture. The protein and RNA distributions between cellular components varied with growth rate. The ribosomal fractions increased with increasing growth rate, as did the RNA:protein ratios in these fractions, in both cultures. Mg-limited bacteria contained little polysaccharide; washed suspensions of such organisms synthesized polysaccharide from glycerol at a low rate as compared with C-limited bacteria. Added Mg stimulated polysaccharide synthesis by Mg-limited bacteria but not by C-limited bacteria. Washed suspensions of bacteria were induced to synthesize -galactosidase. With cultures grown at three different dilution rates, the rates of enzyme synthesis in C-limited bacteria were twice those found with Mg-limited bacteria, though both had equal ribosome contents.

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/content/journal/micro/10.1099/00221287-39-3-355
1965-06-01
2024-12-12
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References

  1. Brock T. D. 1962; Effects of magnesium ion deficiency on Escherichia coli and possible relation to the mode of action of Novobiocin. J. Bact 84:679
    [Google Scholar]
  2. Brody S. 1957; Ribonuclease activity and cellular growth. Biochim. biophys. Acta 24:502
    [Google Scholar]
  3. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J 62:315
    [Google Scholar]
  4. Callow D. S., Pirt S. J. 1956; Automatic control of pH value in cultures of microorganisms. J. gen. Microbiol 14:661
    [Google Scholar]
  5. Dixon M., Webb E. C. 1958 Enzymes London: Longmans Green and Co. Ltd;
    [Google Scholar]
  6. Ecker R. E., Schaechter M. 1963; Ribosome content and the rate of growth of Salmonella typhimurium . Biochim. biophys. Acta 76:275
    [Google Scholar]
  7. Edelman I. S., Ts’o P. O. P., Vinograb J. 1960; The binding of magnesium to microsomal nucleoprotein and ribonucleic acid. Biochim. biophys. Acta 43:393
    [Google Scholar]
  8. Elsworth R., Meakin L. R. P., Pirt S. J., Capell C. H. 1956; A two litre scale continuous culture apparatus for microorganisms. J. appl. Bcta 19:264
    [Google Scholar]
  9. Fales F. W. 1951; Assimilation and degradation of carbohydrates by yeast cells. J. biol. Chem 193:113
    [Google Scholar]
  10. Herbert D. 1958; Some principles of continuous culture. In Recent Progress in Microbiology. Symp. 7th int. Congr. Microbiol381 Stockholm: Almquist and Wiksell;
    [Google Scholar]
  11. Herbert D. 1961; The chemical composition of microorganisms as a function of their environment. In Microbiol Reaction to Environment. Symp. Soc. gen. Microbiol 11391
    [Google Scholar]
  12. Herbert D., Elsworth R., Telling R. C. 1956; The continuous culture of bacteria; a theoretical and experimental study. J. gen. Microbiol 14:601
    [Google Scholar]
  13. Hughes D. E. 1951; A press for disrupting bacteria and other micro-organisms. Brit. J. exp. Path 32:97
    [Google Scholar]
  14. Kennell D., Magasanik B. 1962; The relation of ribosome content to the rate of enzyme synthesis in Aerobader aerogenes . Biochim. biophys. Acta 55:139
    [Google Scholar]
  15. Lucas J. M., Schuurs A. H. W. M., Simpson M. V. 1964; Variation in ribosomal activity and in RNA synthesis during logarithmic growth. Biochemistry 3:959
    [Google Scholar]
  16. McCarthy B. J. 1962; Effects of magnesium starvation on the ribosome content of Escherichia coli . Biochim. biophys. Acta 55:880
    [Google Scholar]
  17. Militzer W. E. 1946; Note on the orcinol reagent. Arch. Biochem 9:85
    [Google Scholar]
  18. Ohtaka Y., Uchida K. 1963; The chemical structure and stability of yeast ribosomes. Biochim. biophys. Acta 76:94
    [Google Scholar]
  19. Postgate J. R., Crumpton J. E., Hunter J. R. 1961; The measurement of bacterial viabilities by slide culture. J. gen. Microbiol 24:15
    [Google Scholar]
  20. Postgate J. R., Hunter J. R. 1962; The survival of starved bacteria. J. gen. Microbiol 29:233
    [Google Scholar]
  21. Powell E. O., Errington F. P. 1963; The size of bacteria, as measured with the Dyson image-splitting eyepiece. J. R. microsc. Soc 82:39
    [Google Scholar]
  22. Stickland L. H. 1951; The determination of small quantities of bacteria by means of the biuret reaction. J. gen. Microbiol 5:698
    [Google Scholar]
  23. Sykes J., Tempest D. W. 1965; The effect of magnesium and of carbon limitation on the macromolecular organisation and metabolic activity of Pseudomonas sp., Strain c-1b. Biochim. biophys. Acta in the press
    [Google Scholar]
  24. Tissières A., Watson J. D. 1958; Ribonucleoprotein particles from Escherichia coli . Nature, Lond 182:778
    [Google Scholar]
  25. Wade H. E., Robinson H. K. 1963; Absence of ribonuclease from the ribosomes of Pseudomonas fluorescens . Nature, Lond 200:661
    [Google Scholar]
  26. Webb M. 1949; The influence of magnesium on cell division: 3. The effect of magnesium on the growth of bacteria in simple chemically defined media. J. gen. Microbiol 3:418
    [Google Scholar]
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