1887

Abstract

SUMMARY

Ribosomes and phenol-purified RNA were prepared from and were characterized by sucrose density-gradient centrifugation and sedimentation-velocity centrifugation. The ribosomes extracted from correspond to the 30S, 50S and 70S ribosomes derived from other bacteria. Under appropriate conditions polyribosomes were extracted. The primary ribosomal RNA of corresponds to the 16S particle of other bacteria. was devoid of the 23S ribosomal RNA typically found in other bacteria. Preparations of phenol-purified RNA contain, in addition to 16S RNA, smaller amounts of an RNA component which sediments more slowly than the 16S unit, with a sedimentation coefficient of about 14–15S. Of several other Athiorhodaceae examined, only had an unusual RNA complement like that of

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/content/journal/micro/10.1099/00221287-39-3-311
1965-06-01
2024-12-13
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References

  1. Aronson A. I. 1962; Sequence differences between ribonucleic acids isolated from 30S and 50S ribosomes. J. mol. Biol 5:453
    [Google Scholar]
  2. Aronson A. I. 1963; Nucleotide sequence differences among ribosomal ribonucleic acid fractions and soluble ribonucleic acid fractions from various bacterial species. Biochim. biophys. Ada 72:176
    [Google Scholar]
  3. Aronson A. I., McCarthy B. J. 1961; Studies of E. coli ribosomal RNA and its degradation products. Biophys. J 1:215
    [Google Scholar]
  4. Britten R. J., Roberts R. B. 1960; High resolution gradient sedimentation analysis. Science 131:32
    [Google Scholar]
  5. Kurland C. G. 1960; Molecular characterization of ribonucleic acid from Escherichia coli ribosomes. J. mol. Biol 2:83
    [Google Scholar]
  6. Lascelles J. 1959; Adaptation to form bacterioehlorophyll in Rhodopseudomonas spheroides: changes in activity of enzymes concerned in pyrrole synthesis. Biochem. J 72:508
    [Google Scholar]
  7. Lowry O. H., Rosebrough N. J., Farn A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. J. biol. Chem 193:265
    [Google Scholar]
  8. Petermann M. L., Pavlovec A. 1963; Studies on ribonucleic acid from rat liver ribosomes. J. biol. Chem 238:3717
    [Google Scholar]
  9. Schlessinger D. 1963; Protein synthesis by polyribosomes on protoplast membranes of B. megaterium . J. mol. Biol 7:569
    [Google Scholar]
  10. Schneider W. C. 1945; Phosphorus compounds in animal tissues. 1. Extraction and estimation of desoxypentose nucleic acid and of pentose nucleic acid. J. biol. Chem 161:293
    [Google Scholar]
  11. Schramm G., Gierer A. 1956; Infectivity of ribonucleic acid from tobacco mosaic virus. Nature, Lond 177:702
    [Google Scholar]
  12. Spahr P. F., Tissieres A. 1959; Nucleotide composition of ribonucleoprotein particles from Escherichia coli . J. mol. Biol 1:237
    [Google Scholar]
  13. Yankofsky S. A., Spiegelman S. 1963; Distinct cistrons for the two ribosomal RNA components. Proc. natn. Acad. SciU.S.A 49539
    [Google Scholar]
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