Over 100 histidine-dependent mutants were isolated from strain 655 of Paper chromatography was used to differentiate the mutants into classes according to their ability to accumulate Pauly-positive imidazoles. Classes and did not accumulate any detectable Pauly-positive imidazoles. Mutants of class accumulated -amino-1-ribosyl-4-imidazolecarboxamide, mutants of class accumulated imidazoleglycerol, and mutants of class accumulated imidazoleacetol. Mutants of class accumulated histidinol and were incapable of utilizing exogenous -histidinol. A comparison of the accumulations obtained from mutants of with accumulations obtained from previously characterized mutants of indicates that a similar, if not identical, pathway for the biosynthesis of -histidine is used by these two species.


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