1887

Abstract

SUMMARY

The growth characteristics of cultures treated with dihydrostreptomycin and then freed from extracellular antibiotic before growth had completely stopped, were examined. Growth, measured by extinction, proceeded exponentially, but at slower rates for a time, followed by gradual recovery. The degree of slowing of growth rate was a function of the duration of growth in the presence of a given concentration of dihydrostreptomycin. Comparison of viable colony count data and microscopic observation of such treated cultures showed that the majority of individuals in the populations must grow at the lower rates for two-three generations, after which some organisms cease to multiply and the rest recover. The proportion of organisms in treated populations which eventually ceased to grow was also a function of the duration of treatment. The amount of growth (cell synthesis), which had occurred at the time when onset of recovery became measurable, varied inversely with the % inhibition of growth rate. This suggests that recovery was due to some process not inhibited during the phase of inhibited exponential growth. It is concluded that intracellular dihydrostreptomycin consists of an ‘inhibitory fraction’ at the sites of inhibition, and a non-inhibitory ‘pool’ fraction; that the size of the latter varies between different individuals within a population and that transfer from ‘pool’ to inhibitory sites occurs by a process other than equilibration; e.g. that the factors which govern the uptake into these two phases must be, at least partly, independent. It is suggested that the degree of inhibition of growth rate reflects the extent of combination between antibiotic and inhibition sites at the time when extracellular dihydrostreptomycin is removed and no further uptake into the organisms can occur, and that the complex between dihydrostreptomycin and inhibition sites cannot dissociate to give active antibiotic which could re-enter the ‘pool’.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-39-2-165
1965-05-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/39/2/mic-39-2-165.html?itemId=/content/journal/micro/10.1099/00221287-39-2-165&mimeType=html&fmt=ahah

References

  1. Bretz H. W. 1962; A simple method for estimating slide culture survival. J. Bact 84:1115
    [Google Scholar]
  2. Davies J. E. 1964; Studies on the ribosomes of streptomycin-sensitive and resistant strains of Escherichia coli . Proc. natn. Acad. SciU. S. A. 51659
    [Google Scholar]
  3. Dubin D. T., Davis B. D. 1962; The streptomycin-triggered depolymerization of ribo-nucleic acid in Escherichia coli . Biochem. biophys. Acta 55:793
    [Google Scholar]
  4. Erdös T., Ullmann A. 1959; Effect of streptomycin on the incorporation of amino-acids labelled with carbon-14 into ribonucleic acid and protein in a cell-free system of Mycobacterium. Nature, Lond 183:618
    [Google Scholar]
  5. Flaks J. G., Cox E. C., White J. R. 1962; Inhibition of polypeptide synthesis by streptomycin. Biochem. Biophys. Res. Commun 7:385
    [Google Scholar]
  6. Flaks J. G., Cox E. C., Whitting M. L., White J. R. 1962; Polypeptide synthesis with ribosomes from streptomycin-resistant and dependent Escherichia coli. Biochem. Biophys. Res. Commun 7:390
    [Google Scholar]
  7. Hancock R. 1962a; Uptake of 14C-streptomycin by some microorganisms and its relation to their sensitivity. J. gen. Microbiol 28:493
    [Google Scholar]
  8. Hancock R. 1962b; Uptake of 14C-streptomycin by Bacillus megaterium. J. gen. Microbiol 28:503
    [Google Scholar]
  9. Harris N. D., Whitefield M. 1963; A lethal effect on damaged Escherichia coli associated with the counting technique. Nature, Lond 200:606
    [Google Scholar]
  10. Hurwitz C., Landau J. V., Doppel H. W. 1962; Effect of exposure of Escherichia. coli to streptomycin on ability to undergo cell division. J. Bact 84:1116
    [Google Scholar]
  11. Hurwitz C., Rosano C. L. 1962; Accumulation of label from 14C-streptomycin by Escherichia coli. J. Bact 83:1193
    [Google Scholar]
  12. Hurwitz C., Doppel H. W., Rosano C. L. 1964; Correlation of the in vivo action of streptomycin on survival and on protein synthesis by Mycobacterium fortuitum. J. gen. Microbiol 35:159
    [Google Scholar]
  13. Kogut M., Lightbown J. W. 1963; Streptomycin action and aerobiosis. Biochem. J 89:18
    [Google Scholar]
  14. Kogut M., Lightbown J. W. 1964a; The mode of action of streptomycin. In Experimental Chemotherapy 3 Schnitzer R. J., Hawking F. New York: Academic Press Inc;
    [Google Scholar]
  15. Kogut M., Lightbown J. W. 1964b; Growth of dihydrostreptomycin-treated Escherichia coli after removal of extracellular antibiotic. J. gen. Microbiol 34:x
    [Google Scholar]
  16. Kogut M., Lightbown J. W., Isaacson P. 1965; Streptomycin action and anaero-biosis. J. gen. Microbiol 39:155
    [Google Scholar]
  17. Litwak G., Pramer D. 1957; Absorption of antibiotics by plant cells. III. Kinetics of streptomycin uptake. Arch. Biochem. Biophys 68:396
    [Google Scholar]
  18. Mager J., Benedict M., Artman M. 1962; A common site of action for polyamines and streptomycin. Biochim. biophys. Acta 62:202
    [Google Scholar]
  19. Miles A. A., Misra S. A. 1938; The estimation of the bactericidal power of blood. J. Hyg., Camb 38:732
    [Google Scholar]
  20. Norris K. P., Powell E. O. 1961; Improvements in determining total counts of bacteria. J. R. Microscop. Soc 80:107
    [Google Scholar]
  21. Postage J. R., Crumpton J. E., Hunter J. R. 1961; The measurement of bacterial viability by slide culture. J. gen. Microbiol 24:15
    [Google Scholar]
  22. Pramer D. 1956; Absorption of antibiotics by plant cells. II. Streptomycin. Arch. Biochem. Biophys 62:265
    [Google Scholar]
  23. Schaechter M., Maaløe O., Kjeldgaard N. O. 1958; Dependency on medium and temperature of cell size and chemical composition during balanced growth of Salmonella typhimurium. J. gen. Microbiol 19:592
    [Google Scholar]
  24. Speyer J. F., Lengyel P., Basilio C. 1962; Ribosomal localization of streptomycin sensitivity. Proc. natn. Acad. SciU. S. A 48684
    [Google Scholar]
  25. Spotts C. R. 1962; Physiological and biochemical studies on streptomycin-dependence in Escherichia coli. J. gen. Microbiol 28:347
    [Google Scholar]
  26. Spotts C. R., Stanier R. Y. 1961; Mechanism of streptomycin action on bacteria: a unitary hypothesis. Nature, Lond 192:633
    [Google Scholar]
  27. Szybalski W., Mashima S. 1959; Uptake of streptomycin by sensitive, resistant and dependent bacteria. Biochem. Biophys. Res. Commun 1:249
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-39-2-165
Loading
/content/journal/micro/10.1099/00221287-39-2-165
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error