1887

Abstract

SUMMARY

Many different strains of and members of other species of the genus were induced to lyse by exposure to the proper concentration of mitomycin C, a radiomimetic drug. For some bacilli, induction of lysis occurred only in Casamino acids containing medium but not in nutrient broth + yeast extract medium, whereas other bacilli showed the opposite behaviour. The efficiency of induction was a function of the growth phase of the culture and of the concentration of mitomycin C. Induction of lysis in resulted in the release of oedema factor and phospholipase into the medium. Mitomycin C-induced lysis of was prevented by adding chloramphenicol, actinomycin D, or 5-fluorouracil deoxyriboside up to 40 min. later. There appeared to be no sequential loss of susceptibility to inhibition by these compounds as expected.

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/content/journal/micro/10.1099/00221287-39-1-53
1965-04-01
2022-05-16
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References

  1. Altenbern R. A. 1962; Lysogeny and toxinogeny in Bacillus cereus . Biochem. Biophys. Res. Communs 9:109
    [Google Scholar]
  2. Csuzi S., Kramer M. 1962; Production of a lytic factor by ultraviolet light irradiated cultures of Bacillus cereus . Acta microbiol. hung. 9:297
    [Google Scholar]
  3. Faguet M. 1962; Étude de l’influence inhibitrice de certains composes chimiques sur l’induction de la lyse et la production de phages provoquées par l’irradiation de cultures d’Escherichia coli lysogène par un rayonnement ultraviolet. C. r. hebd. Seanc. Acad. Sci., Paris 255:1810
    [Google Scholar]
  4. Molnar D. M. 1962; Separation of the toxin of Bacillus cereus into two components and non-identity of the toxin with phospholipase. J. Bact 84:147
    [Google Scholar]
  5. Otsugi N., Sekiguchi M., Iijima T., Takagi Y. 1959; Induction of phage formation in the lysogenic Escherichia coli K-12 by mitomycin C. Nature, Lond 184:1079
    [Google Scholar]
  6. Reich E., Shatkin A. J., Tatum E. L. 1961; Bacteriocidal action of mitomycin C. Biochim. biophys. Acta 53:132
    [Google Scholar]
  7. Seaman E., Tarmy E., Marmur J. 1964; Inducible phages of Bacillus subtilis . Biochemistry 3:607
    [Google Scholar]
  8. Thorne C. B. 1962; Transduetion in Bacillus subtilis . J. Bact. 83:106
    [Google Scholar]
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