Two pathways for methionine methyl formation, one cobalamin-dependent and one cobalamin-independent, corresponding to those observed in PA 15 have been found in An initial difficulty in showing the cobalamin-dependent pathway in cell-free extracts proved to be due to the presence of enzymes which caused the removal of adenosylmethionine, a cofactor required for this system. In contrast to PA 15, contains holocobalaminmethyl-transferase even when grown in the absence of cobalamin. When cobalamin is added to the growth medium, the cobalamin-independent pathway is repressed.


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